[OpenSPIM] About the cylindrical lens

[Jan Huisken]

Hi Edgar,

Remember, the cylindrical lens does not form the light sheet. The light sheet is generated by the (well-corrected) illumination objective lens so that, in the case of multi-color excitation, you will not get any focal shift.

Instead, the cylindrical lens just "prevents" the objective lens from focusing the light also in the vertical dimension. If now your cylindrical lens is not color-corrected, the beam coming out of the illumination lens is not perfectly collimated in the vertical dimension across all colors. In the worst case this would lead to a slightly inhomogeneous illumination. We have never had any problems with cylindrical singlet lenses, which are not color corrected. In fact, we use them on most of our setups.

Best
Jan

Dr. Jan Huisken
MPI of Molecular Cell Biology and Genetics
Pfotenhauerstr. 108, 01307 Dresden, Germany

On Jul 2, 2013, at 10:54 AM, Peter Gabriel Pitrone wrote:

> 
> Hello Edgar,
> 
> Your solution of using the simpler cylindrical lens will work well if you don't use other laser
> lines. Stay with the 50 mm focal length, as changing it will change the properties of the
> light-sheet. I'm in the process of testing different lens combinations, and will update the wiki
> accordingly as to what I find.
> 
> Best Regards,
> Pete
> 
> -- 
> Peter Gabriel Pitrone - TechRMS
> Microscopy/Imaging Specialist
> Prof. Dr. Pavel Tomancak group
> Max Planck Institute for
> Molecular Biology and Genetics
> Pfotenhauerstr. 108
> 01307 Dresden
> 
> "If a straight line fit is required, obtain only two data points." - Anon.
> 
> 
> On Fri, June 28, 2013 6:26 pm, skf-ee at ipt.rwth-aachen.de wrote:
> <|> Hi dear All,
> <|>
> <|> I just have a problem:
> <|> Since more than 4 weeks ago I ordered the thorlabs parts for the OpenSPIM, the only one is
> <|> missing is the cylindrical lens (it was supposed to arrive like two weeks ago). I just
> <|> contact them and the problem is that they don't have in stock the ACY254-050-A; they 
> <|> would need to manufacture it and that would take a little long time.
> <|>
> <|> So they propose to use the ACY254-075-A, the only difference of this lens is that the focal
> length
> <|> is 75 mm
> <|> instead of 50 mm. I can see from the Open SPIM design that maybe would be possible to use this
> <|> lens
> <|> if it is moved 25 mm closer to the first two achromatic doublets used to expand the beam in
> <|> comparison
> <|> to the position of the 50 mm focal length lens. I am not an optics expert but maybe it can work.
> <|>
> <|> Anothe solution I found out is to use the  LJ1695RM-A, a cylindrical plano-convex lens from
> <|> Thorlabs with focal length 50 mm,
> <|> AR coating 400-750 nm but with the disadvantage that it is not
> <|> Achromatic, http://www.thorlabs.de/thorproduct.cfm?partnumber=LJ1695RM-A. So if I will use only
> <|> monochromatic illumination sources maybe is not a big deal to use this lens.
> <|>
> <|> So, what do you think is the best solution? I would really appreciate if someone can give me
> <|> her/his advice.
> <|>
> <|> Kind regards.
> <|> Edgar Escobar Nieto
> <|> _________________________________________________________________________
> <|>
> <|> Fraunhofer-Institut für Produktionstechnologie IPT
> <|> Edgar Escobar Nieto
> <|>
> <|>
> <|>
> <|>
> <|> Steinbachstraße 17
> <|> 52074 Aachen
> <|>
> <|> skf-ee at ipt.rwth-aachen.de
> <|> http://www.ipt.fraunhofer.de
> <|> _________________________________________________________________________
> <|> _______________________________________________
> <|> OpenSPIM mailing list
> <|> OpenSPIM at openspim.org
> <|> http://openspim.org/mailman/listinfo/openspim
> <|>
> 
> 
> 
> _______________________________________________
> OpenSPIM mailing list
> OpenSPIM at openspim.org
> http://openspim.org/mailman/listinfo/openspim

-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://openspim.org/pipermail/openspim/attachments/20130702/22f4ce4c/attachment-0002.html>