<html><head></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">Hi Edgar,<div><br></div><div>Remember, the cylindrical lens does not form the light sheet. The light sheet is generated by the (well-corrected) illumination objective lens so that, in the case of multi-color excitation, you will not get any focal shift.</div><div><br></div><div>Instead, the cylindrical lens just "prevents" the objective lens from focusing the light also in the vertical dimension. If now your cylindrical lens is not color-corrected, the beam coming out of the illumination lens is not perfectly collimated in the vertical dimension across all colors. In the worst case this would lead to a slightly inhomogeneous illumination. We have never had any problems with cylindrical singlet lenses, which are not color corrected. In fact, we use them on most of our setups.</div><div><br></div><div>Best</div><div>Jan</div><div><br><div>
<span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: medium; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: medium; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: medium; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><div style="font-size: 11px; ">Dr. Jan Huisken</div><div style="font-size: 11px; "><b>MPI of Molecular Cell Biology and Genetics</b></div><div style="font-size: 11px; ">Pfotenhauerstr. 108, 01307 Dresden, Germany</div></div></span></div></span></span>
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<br><div><div>On Jul 2, 2013, at 10:54 AM, Peter Gabriel Pitrone wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite"><div><br>Hello Edgar,<br><br>Your solution of using the simpler cylindrical lens will work well if you don't use other laser<br>lines. Stay with the 50 mm focal length, as changing it will change the properties of the<br>light-sheet. I'm in the process of testing different lens combinations, and will update the wiki<br>accordingly as to what I find.<br><br>Best Regards,<br>Pete<br><br>-- <br>Peter Gabriel Pitrone - TechRMS<br>Microscopy/Imaging Specialist<br>Prof. Dr. Pavel Tomancak group<br>Max Planck Institute for<br>Molecular Biology and Genetics<br>Pfotenhauerstr. 108<br>01307 Dresden<br><br>"If a straight line fit is required, obtain only two data points." - Anon.<br><br><br>On Fri, June 28, 2013 6:26 pm, <a href="mailto:skf-ee@ipt.rwth-aachen.de">skf-ee@ipt.rwth-aachen.de</a> wrote:<br><|> Hi dear All,<br><|><br><|> I just have a problem:<br><|> Since more than 4 weeks ago I ordered the thorlabs parts for the OpenSPIM, the only one is<br><|> missing is the cylindrical lens (it was supposed to arrive like two weeks ago). I just<br><|> contact them and the problem is that they don't have in stock the ACY254-050-A; they <br><|> would need to manufacture it and that would take a little long time.<br><|><br><|> So they propose to use the ACY254-075-A, the only difference of this lens is that the focal<br>length<br><|> is 75 mm<br><|> instead of 50 mm. I can see from the Open SPIM design that maybe would be possible to use this<br><|> lens<br><|> if it is moved 25 mm closer to the first two achromatic doublets used to expand the beam in<br><|> comparison<br><|> to the position of the 50 mm focal length lens. I am not an optics expert but maybe it can work.<br><|><br><|> Anothe solution I found out is to use the LJ1695RM-A, a cylindrical plano-convex lens from<br><|> Thorlabs with focal length 50 mm,<br><|> AR coating 400-750 nm but with the disadvantage that it is not<br><|> Achromatic, <a href="http://www.thorlabs.de/thorproduct.cfm?partnumber=LJ1695RM-A">http://www.thorlabs.de/thorproduct.cfm?partnumber=LJ1695RM-A</a>. So if I will use only<br><|> monochromatic illumination sources maybe is not a big deal to use this lens.<br><|><br><|> So, what do you think is the best solution? I would really appreciate if someone can give me<br><|> her/his advice.<br><|><br><|> Kind regards.<br><|> Edgar Escobar Nieto<br><|> _________________________________________________________________________<br><|><br><|> Fraunhofer-Institut für Produktionstechnologie IPT<br><|> Edgar Escobar Nieto<br><|><br><|><br><|><br><|><br><|> Steinbachstraße 17<br><|> 52074 Aachen<br><|><br><|> <a href="mailto:skf-ee@ipt.rwth-aachen.de">skf-ee@ipt.rwth-aachen.de</a><br><|> <a href="http://www.ipt.fraunhofer.de">http://www.ipt.fraunhofer.de</a><br><|> _________________________________________________________________________<br><|> _______________________________________________<br><|> OpenSPIM mailing list<br><|> <a href="mailto:OpenSPIM@openspim.org">OpenSPIM@openspim.org</a><br><|> <a href="http://openspim.org/mailman/listinfo/openspim">http://openspim.org/mailman/listinfo/openspim</a><br><|><br><br><br><br>_______________________________________________<br>OpenSPIM mailing list<br><a href="mailto:OpenSPIM@openspim.org">OpenSPIM@openspim.org</a><br>http://openspim.org/mailman/listinfo/openspim<br></div></blockquote></div><br></div></body></html>