[OpenSPIM] laser for openSPIM

Monika Pawłowska m.pawlowska at nencki.gov.pl
Mon Feb 12 03:30:36 CST 2018


You can find cheaper lasers with good beam quality, but if you have a  
laser which can't be controlled from uManager, your sample will be  
illuminated all the time, also during moving or timelapse waiting periods.  
For some samples this will be a problem.

However, if that's acceptable for you, in the first version of my setup I  
was able to make a cheap diode laser work by focusing it onto a small  
pinhole. It's a similar solution to the fiber one, but somewhat cheaper.  
However, whether you use pinhole or fiber, you'll need a very precise XY  
positioner for effective coupling which is also not cheap (I was using a  
borrowed one).

Regards,
Monika Pawłowska

W dniu .02.2018 o 08:40 Emmanuel DUFOUR <quelleordure at gmail.com> pisze:

> Hello
> The laser parts listed in openSPIM are expensive (7000 euros).
> Is there a way to get cheaper lasers work  ?
> I've been trying to make a very small focus point with a cheap red laser  
> diode without success. I often see several points instead of one. And I  
> cant >make the point(s) smaller than 0.1 mm .
> But I dont know if it's because of the cheap lenses I use, or the  
> aperture that is too big, or the diode itself (transverse mode pattern  
> ?).
> So, what are the bare requirements for the laser in order to make it  
> focus to a size of about 10 um ?
>
> Thanks for your help
> Emmanuel DUFOUR
>



-- 
Monika Pawłowska
Nencki Institute
Laboratory of Neurobiology
3, Pasteur Street
02-093 Warsaw, Poland
phone: (+48) 22 5892 382
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