[OpenSPIM] OpenSPIM Digest, Vol 51, Issue 7

[David Potter]

Dave,

I cut a spacer from shim stock (thin sheet of metal; find at a hobby store)
that took up the empty space. Brass is probably best as the copper in the
brass is a bit anti-bacterial. You can see I didn't follow my own advice
and made mine out of "stainless" steel. It's what I had at the time. Cut
the right length. Roll it into a tube that'll fit the holder. Perhaps put a
crimp at the top so it doesn't fall through. Cheap and cheerful.

I also use a foam stopper like you have in your image though I only use it
for replacing the syringe in the same location if I remove it for any
reason.

Nice job on the belt drive.

Cheers,

- David

David Potter
Lattice Light Sheet Microscopy Manager
Monash Micro Imaging - Advanced Optical Microscopy
Monash University Clayton, VIC 3800, Australia



On 30 November 2017 at 05:00, <openspim-request at openspim.org> wrote:

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> Today's Topics:
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>    1. How do you mount the sample syringe so that it fits       tight?
>       (dave dowless)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 29 Nov 2017 10:08:05 -0500
> From: dave dowless <ddowless at gmail.com>
> To: openspim at openspim.org
> Subject: [OpenSPIM] How do you mount the sample syringe so that it
>         fits    tight?
> Message-ID:
>         <CAM=c-cCL=emfpcCJho-URAQo0jUr2Ao=UQznLmGoAqC7jEJn6
> w at mail.gmail.com>
> Content-Type: text/plain; charset="utf-8"
>
> Hi,
>
> This is my first build. How do folks mount the sample syringe itself?
>
> My syringe isn't tight. It rocks and slips.
>
> Here's a pic of my hybrid Picard Industries stages
> <https://i.imgur.com/9xkyyFS.jpg>
>
> That's a standard Picard twister set up to spin the syringe.
>
> How do you lock down the syringe?
>
> They use foam
> <http://openspim.org/images/thumb/f/fc/Light_Sheet_
> Calibration-1.jpg/800px-Light_Sheet_Calibration-1.jpg>for
> the calibration mirror in the OpenSpim manual.
>
> Is that what folks do? Foam?
>
> Specific syringes?
>
> Any help?
>
> Dave
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> End of OpenSPIM Digest, Vol 51, Issue 7
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