[OpenSPIM] fluorophores for alignment

[Dr S. Hoehn]

Thanks everyone for the advice!

Best wishes,
Stephanie

On 2017-06-15 17:28, Jon Daniels wrote:
> I routinely use a Zebra Mildliner highlighter dipped in water to give
> micron-scale fluorescent blobs that diffuse randomly like beads. I
> have yet to find a Zebra Mildliner variety that doesn't have this
> useful property (most highlighter pens just give a uniform dye, and
> sometimes it is very tough to wash out, unlike fluorescein). I'm
> usually using 488nm; once I tried at 640nm and it was pretty dim but
> maybe choosing another color would have solved the problem (I haven't
> investigated relative signal from different colors of the Zebra
> Mildliner).
> 
> Good luck!
> Jon
> 
> -------------------------------------------
> Jon Daniels
> Applied Scientific Instrumentation
> Phone: (541) 461-8181 x118
> -------------------------------------------
> 
>> I all you want to see is the laser beam in the field of view than
> you're good
>> with water and no emission filter.
>> 
>> Best,
>> Michael
>> 
>> > On Jun 15, 2017, at 11:19 AM, Feinstein, Timothy N <tnf8 at pitt.edu>
> wrote:
>> >
>> > We can see the laser well enough using about 1% phenol red in
> water, at
>> least with 488 and 561 nm lasers. I have not tried 647 nm. This is
> for the
>> alignment technique where you remove the cylinder lens correct?
>> >
>> > Best,
>> >
>> >
>> > Tim
>> >
>> > Timothy Feinstein, Ph.D.
>> > Research Scientist
>> > University of Pittsburgh Department of Developmental Biology
>  > >
>> >
>> > On 6/15/17, 10:30 AM, "OpenSPIM on behalf of Dr S. Hoehn"
>> <openspim-bounces at openspim.org on behalf of S.Hoehn at damtp.cam.ac.uk>
> wrote:
>> >
>> > Hello,
>> >
>> > Can anyone recommend (cheap) fluorophores that are fluorescent in
> water
>> > for alignment purposes?
>> >
>> > We'd specifically need one for a 637nm laser, but fluorophores for
> the
>> > 488nm and 534nm laser line would also be helpful.
>> >
>> > Thanks a lot.
>> >
>> > Best wishes,
>> > Stephanie
>> >
>> >
>> > Dr. Stephanie Hoehn
>> > DAMTP, Biological Physics
>> > Centre for Mathematical Sciences
>  > > Wilberforce Road
>> > Cambridge
>> > CB3 0WA
>> > United Kingdom
>> > Office Tel: 01223 337859
>> > Office: H0.03
>> >
>> >
>> >
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>> > OpenSPIM at openspim.org
>> >
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