[OpenSPIM] distance telescope illumination objective
Weber, Michael
Michael_Weber at hms.harvard.edu
Fri Jun 9 09:15:14 CDT 2017
Hi Stephanie,
Every distance after the cylindrical lens is critical. Moving the sample chamber with respect to the telescope will shift the position of the light sheet waist. If you want to increase the distance between telescope and chamber you could build the telescope out of two lenses with longer focal lengths but the same ratio.
Best,
Michael
From: Dr S. Hoehn<mailto:S.Hoehn at damtp.cam.ac.uk>
Sent: Friday, June 9, 2017 9:43 AM
To: OpenSPIM at openspim.org<mailto:OpenSPIM at openspim.org>
Subject: [OpenSPIM] distance telescope illumination objective
Dear all,
do I understand correctly that there has to be a specific distance
between the last telescope lens and the illumination objective?
So I could NOT move the sample chamber further away from the telescope?
I was thinking about doing this since we have a wider bread board and a
longer path would make it easier to align the laser.
Thank you,
Stephanie
Dr. Stephanie Hoehn
DAMTP, Biological Physics
Centre for Mathematical Sciences
Wilberforce Road
Cambridge
CB3 0WA
United Kingdom
Office Tel: 01223 337859
Office: H0.03
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