[OpenSPIM] Alignment of light sheet

[Wiebke Jahr]

Hello Kathleen,

attached is an image of the tools that you can use for alignment: left a 
homebuild version (without grids), right a professional version 
(unfortunately not for sale).

The Edmund Multi-Grid test is based on a standard glass coverslip; the 
patterns are a very thin metal coating. You can cut the glass coverslip 
with a diamond pen, but getting the small pieces you need is somewhat 
tricky. Apparently it works better when you first make all the cuts 
needed, then break the coverslip.

The test target can be reused. Ideally, just use distilled water in your 
chamber while aligning. If you need to use buffer, inspect the glue 
regularly to make sure it does not dissolve (may damage your objektives 
if it's in solution and then deposits on them) and rinse the test target 
afterwards.

Best, Wiebke

-- 
------------------------------------------------------------
Wiebke Jahr
PhD Student
Tel. +49 351 210-2768

Huisken Lab,
Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauer Str. 108, 01307 Dresden
------------------------------------------------------------

On 31/07/2017 17:22, Kathleen Chen wrote:
> Hello OpenSPIM members,
>
> I was wondering how other members performed the alignment of the light 
> sheet.
>
> The wiki suggests the use of a portion of the Edmund Optics Multi-Grid 
> test grid glued to a syringe with a small mirror. However, there are 
> not many details regarding the making of this test grid/mirror syringe 
> part so I have been having some trouble with the alignment.
>
> This method seems to require cutting out the grid from the EO part, 
> which from the images seems to be metal. Also, can this grid/mirror 
> syringe setup be reused or do we need to use a new grid every time the 
> sheet needs to be aligned? Have others experienced any trouble with 
> this or do you have any tips/suggestions?
>
> Thank you,
> Kathleen Chen
>
>
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