[OpenSPIM] Fluorescent solution for light sheet alignment

Nikita Vladimirov nvladimus at gmail.com
Tue Aug 22 07:23:29 CDT 2017


Yes, I did, it worked nicely with fluorescein, for finding the beam waist
in the field of view, and checking the XY tilt.

Nikita Vladimirov

On Tue, Aug 22, 2017 at 12:28 PM, Emmanuel Reynaud <emmanuel.reynaud at ucd.ie>
wrote:

> Hi,
>
> Did anybody ever used a chamber filled with fluorescent solution (e.g.
> fluorescein) to align the light sheet? Got a request from another group who
> planned to use such method to align detection vs illumination objectives?
>
> Any ideas welcome,
>
> Emmanuel
>
> *Dr Emmanuel G. Reynaud*
> Principal Investigator, Integrative Biology
> Conway Institute of Biomolecular and Biomedical Science
> UCD Centre for Biomedical Engineering (http://www.ucd.ie/
> biomedicalengineering/)
>
> e-mail: emmanuel.reynaud at ucd.ie <matthias.wilm at ucd.ie>
> Website:http://cellbiology.ucd.ie/
>
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>
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