[OpenSPIM] better 4D-Stages for openSPIM

[Monika Pawłowska]

Dear Florian,

I spent quite some time on this issue when first building my setup. First,  
bad news:
-I haven't found any other ready-made 4D stage
-do I understand correctly that the OpenSPIM plugin only works with Picard?

But one can get a XYZ stage and then somehow add an arm for the sample and  
rotation, for example by using the R part of the Picard 4D if you already  
have it. And then, if you want to use uManager, pick something compatible  
with uManager. I recently abandoned my Picard and picked a XYZ stage by  
Standa - they have a good choice of ranges, I chose 25 mm from the 8MT173  
series. It works fine with uManager. I only have manual rotation right  
now, because I don't use multiview. Thorlabs also has a compact XYZ stage.  
All the other I found were much more expensive.

I hope this helps.

Monika

W dniu .10.2016 o 17:27 Florian Vollrath <florian.vollrath at brain.mpg.de>  
pisze:

> Hi all,
> I am really glad for my participation of the EMBO light sheet course  
> this year. I learned really a lot. This knowledge now led to the point  
> that we want to build our own light sheet microscope in our institute  
> (MPI for Brain Research in Frankfurt).
> We will begin with a simple openSPIM setup and put in more advanced  
> things in the future. The reason why I am writing here is that the  
> recommended 4D stage is not as good as we would like it to be. Does  
> anybody know of good commercial 4D stages with a range of 1-2 cm or even  
> more and a resolution as good as possible (>500nm)? Or would you  
> recommend to build one on our own? I would be very thankful for any help.
> Kind regards,
> Florian
>


-- 
Dr Monika Pawłowska
Nencki Institute
02-093 Warsaw
Pasteura 3
Poland