[OpenSPIM] Issue with shutter/laser

Ulrik Günther guenther at mpi-cbg.de
Fri Nov 18 06:27:55 CST 2016


Hi Alexis,


unfortunately, the corelog doesn’t shed too much light onto the problem.
However, you could do the following:


1) Update to a more recent MM version (corelog indicates you’re using 1.4.18, which is a little dated… the problem may have been fixed in the meantime).


2) Try narrowing the problem down to a specific device by creating a config where you replace stage, shutter, cam one-by-one with the respective option from the DemoCamera class and see if that config works.


cheers!


ulrik
> On Nov 18, 2016, at 12:55 PM, Alexis Maizel <alexis.maizel at cos.uni-heidelberg.de> wrote:
> 
> 
> Sorry for the missing CoreLog… here it is.
> It happens in general.
> 
> 
> Best,
> 
> 
> Alexis
> 
> 
> 
>> 
>> 
>> On 18 Nov 2016, at 11:40, Ulrik Günther <guenther at mpi-cbg.de> wrote:
>> 
>> 
>> Hi Alexis,
>> 
>> 
>> does this only happen with certain imaging configurations, or in general when using Multi-D?
>> Also, the corelog is not attached to your email (only your GPG signature). Could you resend it please?
>> 
>> 
>> cheers!
>> 
>> 
>> ulrik
>> 
>> 
>> 
>> 
>> 
>>>> Ulrik Günther, Dipl.-Phys.
>> PhD Student
>> MOSAIC Group & Tomancak Lab, Center of Systems Biology Dresden
>> Max Planck Institute of Molecular Cell Biology and Genetics
>> Pfotenhauerstr. 108, D-01307 Dresden, Germany
>>> 
>>> 
>>> On Nov 18, 2016, at 11:33 AM, Alexis Maizel <alexis.maizel at cos.uni-heidelberg.de> wrote:
>>> 
>>> 
>>> Hi everyone,
>>> 
>>> 
>>> I recently exchanged the computer driving our T-openSPIM. On the new computer, almost everything is working perfectly. Very strangely, I get an error when I want to trigger an image acquisition using MicroManager’s "Multi-Dimensional Acquisition” module: "Error in device "Cobolt": Invalid state (position) requested (12)”.
>>> I have no problem to acquire image otherwise (live mode, SPIMAcquisition plugin). The problem only seems to be with the MM MultiD module.
>>> 
>>> 
>>> I attach the Corelog.
>>> 
>>> 
>>> I have of course, created a brand new config file and reinstalled the drivers for the laser but that did not solve the issue. I am totally clueless, as this config had been working seamlessly for ages. When migrating to the new computer I took care of zipping Fiji/MM bundle and transfer it "as is” to the new machine.
>>> 
>>> 
>>> If anyone has an idea of what could have gone wrong…
>>> > 
>>> 
>>> 
>>> Many thanks in advance.
>>> 
>>> 
>>> 
>>> 
>>> 
>>> 
>>> 
>>> 
>>> 
>>> 
>>> 
>>>>>> Prof. Dr. Alexis Maizel
>>> Developmental Plasticity in Plants
>>> Center for Organismal Studies
>>> University of Heidelberg
>>> Im Neuenheimer Feld 230
>>> 69120 Heidelberg
>>> Germany
>>> 
>>> 
>>> Office: +49 6221 54 64 56
>>> Fax: +49 6221 54 64 24
>>> Cell: +49 151 40 10 23 28
>>> Email: alexis.maizel at cos.uni-heidelberg.de
>>> URL: www.maizel-lab.org
>>> Orcid: http://orcid.org/0000-0001-6843-1059
>>> _______________________________________________
>>> OpenSPIM mailing list
>>> OpenSPIM at openspim.org
>>> http://openspim.org/mailman/listinfo/openspim
>>> 
> 
> 
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