[OpenSPIM] Optical abberations

[Nikita Vladimirov]

Hi, Matt,
The image seems to have really strong astigmatism, and the optical axis is
way off center. The lens you use has f=25mm, it is a far cry from what the
tube lens is expected to be, f=180 mm for Olympus objective. You cannot
expect any decent quality from a tube lens which is so far from what the
system design (objective - tube lens). You need a proper tube lens, or at
least an achromat with similar focal distance to have a decent image
quality.
Best,
Nikita



On Wed, Nov 16, 2016 at 11:52 AM, FORD Matthew <Matthew.Ford at ed.ac.uk>
wrote:

> Hi OpenSPIMers,
>
>
> I am in the process of building an open spim system based on the original
> open spim. I've tried to image some fluorescent beads but am seeing an odd
> effect where beads around the edge are being distored with the curvature  of
> the lens (I have attached an image). We are using a * LA1951-A - N-BK7
> Plano-Convex Lens* as a tube lens rather than the olympus U-TLU. I was
> wondering if the tube lens is what is causing the abborations but I am
> fairly new to optics so am not sure. If anyone has any advice on what might
> be causing the abborations I would lvoe to hear your suggestions as I'm a
> bit stuck.
>
>
> Thanks in advance for your advice,
>
>
> Matt
>
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> Scotland, with registration number SC005336.
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