[OpenSPIM] Stradus Versalase for OpemSPIM

[Feinstein, Timothy N]

Hi Matt, 

We use a 488/561 Versalase with an ESio TTL controller to enhance
switching speed.  Our setup largely followed a design that was generously
provided by Johannes Girstmair in the Telford lab at University College
London, with a few modifications to fit our needs.  He provided me
extensive help that I can pass along if he has not done already.

Overall it works quite well.  If you use a TTL controller, you will want
to add the following to the Configuration Settings window in
Micro-Manager: 

Laser_[A/B/C/D]_LaserEmission
Laser_[color]_DigitalPeakPowerSetting
TTL_State (I rename this setting 'color' and name the TTL states that
result in the desired outputs 'green', 'red' and 'off'.  This lets me set
multi-channel images by assigning the 'color' variable in Multi-D
Acquisition)

Turn on DigitalModulation in the Device Property Browser and you should be
set.  You have to switch LaserEmission on each time you boot up MM and if
you use a TTL controller PeakPower will determine the laser intensity.

Blanking mode will theoretically help acquire faster with minimized light
exposure.  However I have not got that to work; Micro-Manager's
auto-shutter seems to do the job well enough.

All the best, 


Tim

Timothy Feinstein, Ph.D.
Research Scientist
University of Pittsburgh Department of Developmental Biology





On 2/23/16, 5:05 AM, "openspim-bounces at openspim.org on behalf of PEARSON
Matthew" <openspim-bounces at openspim.org on behalf of
matthew.pearson at igmm.ed.ac.uk> wrote:

>Hi all,
>
>I was wondering if anyone is using a Stradus Versalase with OpenSPIM and
>if so i'm interested in knowing what properties of the laser are required
>and included in the micro-manager config group/preset.  So far i have
>created a Micro-Manager config file that contains all the required SPIM
>devices.
>
>Thanks,
>
>Matt
>
>--
>Matt Pearson
>Microscopy Facility
>MRC Human Genetics Unit
>IGMM
>University of Edinburgh
>Crewe Road
>EH4 2XU
>
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