[OpenSPIM] Is anyone using digital scanning to reduce banding?

[Monika Pawłowska]

Re: removing stripes in post-processing I tried this plugin:

http://www.math.univ-toulouse.fr/~weiss/PageCodes.html

and it's not bad, but lately stopped working for me after updating fiji.

Monika

W dniu .04.2016 o 16:13 Feinstein, Timothy N <tnf8 at pitt.edu> pisze:

> Hello all,
>
> I found the Selzer group's solution (Keller et al. 2013) quite  
> interesting
> and would love to know if anyone had a positive experience doing that.
> The banding from my basic openSPIM is definitely a problem for
> segmentation and analysis.
>
> As far as I can tell fixing the banding will mostly involve buying a scan
> mirror.  I don't have a quote yet for the Cambridge VM500 scanning mirror
> that the Selzer group used but its ThorLabs equivalent (#GVS101?) costs
> about $1k.  That seems reasonable and not that hard for regular folks to
> implement.
>
> In related news I just noticed that the TeraStitcher plugin for Vaa3D now
> has an option to fight banding in light sheet data.  I have not tested it
> yet but potentially that feature could be useful.  I will just post my
> usual warning for folks trying TeraStitcher for the first time: it
> stitches really well but it could be a hair more user-friendly.
>
> Best,
>
>
> Tim
>
> Timothy Feinstein, Ph.D.
> Research Scientist
> University of Pittsburgh Department of Developmental Biology
>
>
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-- 
Dr Monika Pawłowska
Nencki Institute
02-093 Warsaw
Pasteura 3
Poland