[OpenSPIM] OpenSPIM and optically cleared 3D tissue

[Peter Gabriel Pitrone]

with the exeption of the "Meso-lens" from Prof. Brad Amos... But I'm sure it uses glue, and you probably can not afford it.

http://www2.mrc-lmb.cam.ac.uk/research/technology-transfer/mesolens-microscopy/



----- Original Message -----
From: "Peter Gabriel Pitrone" <pitrone at mpi-cbg.de>
To: "Jamie Flynn" <Jamie.Flynn at uon.edu.au>
Cc: openspim at openspim.org
Sent: Friday, October 23, 2015 10:14:06 AM
Subject: Re: [OpenSPIM] OpenSPIM and optically cleared 3D tissue

Hey Jamie,

I would have someone design the lenses (and therefor chamber as well) for you made in a way the is fluid/liquid proof WITHOUT using glue, also keeping in mind the absolute largest (NA in terms of detection lens, and) working distance possible so you could put in bigger tissues. I doubt that there is a objective on the market that would meet your requirements.

Regards,
Pete

----- Original Message -----
From: "Jamie Flynn" <Jamie.Flynn at uon.edu.au>
To: openspim at openspim.org
Sent: Friday, October 23, 2015 1:58:29 AM
Subject: [OpenSPIM] OpenSPIM and optically cleared 3D tissue

Hi everyone, 

We’re building a dual illumination OpenSPIM at the Hunter Medical Research Institute in Newcastle, Australia. Our ai m is to have the scope and analysis pipeline used as a core facility for lab groups to image 3D tumour cell cultures, developing embryos and most importantly for us, optically cleared 3D tissue samples (e.g. CLARITY, CUBIC, 3DISCO, Scale etc.). Imaging cleared 3D samples would be an interesting avenue for OpenSPIM and it’d be great to hear about any hardware modifications or mounting protocols others may have used to do it. 

If anyone wants to try clearing out their own tissue samples, my colleagues and I put together a handbook that describes the reagents, equipment and detailed protocols for ‘Passive CLARITY’. We compiled the protocols outlined in Tomer et al. Nat. Prot. 9(7) 2014 and Yang et al. Cell (158) 2014 and added in our own refinements (after much trial and error!). It’s a very simple technique and has worked really well in our hands. Here’s the link. Download it to your desktop in case the link gets broken: 

https://drive.google.com/open?id=0BzIZDgZFR2srNFk2dHFqT1UycFk 

All the best 
-------------------- 
Jamie Flynn (PhD) 
University of Newcastle 
Hunter Medical Research Institute 
Room 3613 level 3 east 
1 Kookaburra Circuit, New Lambton Heights 
NSW 2305, Australia. 
pH: +612 40420468 
Mobile: +61 416003787 
Email: jamie.flynn at uon.edu.au 


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