[OpenSPIM] Image Stitching Best Practices

m.pawlowska at nencki.gov.pl m.pawlowska at nencki.gov.pl
Mon Jun 29 08:37:28 CDT 2015


Hi Timothy,

I heard about TeraStitcher and I tried to use it on my SPIM data, but I 
got stuck. TeraStitcher expects a certain format of data, OpenSPIM data 
is quite different, so either the data has to be converted or 
TeraStitcher settings changed, but I failed at it. Could you describe in 
more detail how you do it? You mention a "cheat sheet" in the other 
message.

Regards,
Monika

W dniu 2015-06-29 15:18, Feinstein, Timothy N napisaƂ(a):
> Hi Gabriel,
> 
> Among the open source options I have had great results with the
> TeraStitcher plugin for Vaa3d. See my message about it in another
> forum here:
> 
> http://lists.umn.edu/cgi-bin/wa?A2=ind1411&L=CONFOCALMICROSCOPY&P=R10149
> [1]
> 
> Best,
> 
> Tim
> 
> Timothy Feinstein, Ph.D.
> Research Scientist
> University of Pittsburgh Department of Developmental Biology
> 
>  From: Gabriel Davis Jones <gabriel.jones at florey.edu.au>
>  Date: Saturday, June 27, 2015 at 1:18 AM
>  To: "openspim at openspim.org" <openspim at openspim.org>
>  Subject: [OpenSPIM] Image Stitching Best Practices
> 
> Hi All,
> 
> We are currently imaging large volumes that require us to acquire
> multiple stacks of the same angle.
> Is there currently a best way to stitch images acquired OpenSPIM?
> Should we use the grid/collection stitching plugin or is there a
> better way to go about it?
> 
> Thanks,
> 
> Gabriel
> 
> Links:
> ------
> [1]
> http://lists.umn.edu/cgi-bin/wa?A2=ind1411&L=CONFOCALMICROSCOPY&P=R10149
> 
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