[OpenSPIM] Problems with Image Processing

[Windell, Dylan]

Dear All,

I am very new to OpenSPIM and do not have previous experience with regards to coding or ImageJ so I do apologise for all the basic questions.

I have managed to take some practise stack image stacks at 6 angles (300 images - ~2-4GB) but they do not open normally in Image J and the program just says this:

Reading heading file header (Bio-Formats Import Options listing all series), Cancelling thumbnail generation, Import preparations complete - which is followed by nothing.

When I tried pre-processing the images using the macro I used the following adaptions to the code as I only have 1 time-point:

for ( t = 1; t <= 1; t++ ) {
    for ( a = 0; a <= 5; a++ )

As I have Windows 7 64bit, I changed the directory to this:
open("E:\\SPIM_Images\\<file:///\\E:\SPIM_Images\>....spim_TL"

I also attached an exception message I received when trying to process the files.

I must be doing something very wrong and would greatly appreciate any help.

Thank you very much,

Dylan

Dylan Windell
Postgraduate Researcher
College<http://business-school.exeter.ac.uk/research/areas/centres/isr/> of Life and Environmental Sciences
University of Exeter, Geoffrey Pope
Stocker Road, Exeter EX4 4QD, UK
phone: +44 (0) 7596725056
email: dw388 at exeter.ac.uk<mailto:dw388 at exeter.ac.uk>

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