[OpenSPIM] OpenSPIM Digest, Vol 22, Issue 13

Fri Feb 20 13:30:51 CST 2015

Hi David,

At the moment I run an OpenSPIM in a L-mode but I want it upgrade it to 
a T-SPIM. Therefore I integrated already the sample chamber for dual 
side illumination.  Once I'll have the money for the parts of the second 
illumination path and a shutter system I'll go for it.
I uploaded a photo of the chamber that you get an impression 
(http://openspim.org/Who_has_an_OpenSPIM%3F). Pete Pitrone designed this 
master piece and, as Michael already said, he might have it as 3D vector 
graphic file.

Best,
Carsten

Am 20.02.2015 um 19:00 schrieb openspim-request at openspim.org:
> Send OpenSPIM mailing list submissions to
> 	openspim at openspim.org
>
> To subscribe or unsubscribe via the World Wide Web, visit
> 	http://openspim.org/mailman/listinfo/openspim
> or, via email, send a message with subject or body 'help' to
> 	openspim-request at openspim.org
>
> You can reach the person managing the list at
> 	openspim-owner at openspim.org
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of OpenSPIM digest..."
>
>
> Today's Topics:
>
>     1. Re: Information on T-SPIM construction (Michael Weber)
>     2. Re: Information on T-SPIM construction (David Burk)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 20 Feb 2015 13:40:28 +0100
> From: Michael Weber <weber at mpi-cbg.de>
> To: David Burk <davidburk1972 at gmail.com>
> Cc: openspim at openspim.org
> Subject: Re: [OpenSPIM] Information on T-SPIM construction
> Message-ID: <10BEB2B8-54D6-4275-BA4A-B88AAA8DE06C at mpi-cbg.de>
> Content-Type: text/plain; charset="us-ascii"
>
> Hey David,
>
> Pete might have a step file for the 3-objective chamber. And yes, in principle you just need two arms and a 50/50 splitter, but there are some limitations. Unless you add shutters or AOTFs, you simultaneously illuminate from both sides. Depending on the sample, this might not be ideal, as you get an overlay of "sharp" and "blurry" images. This typically gets worse with larger samples. You can add manual shutters to be able to choose the side of illumination. If you want to be able to quickly record images with sequential double-sided illumination, you need to integrate shutters/AOTFs into the software.
>
> Best,
> Michael
>
> On Feb 18, 2015, at 8:41 PM, David Burk <davidburk1972 at gmail.com> wrote:
>
>> I apologize in advance if the answer to my question is somewhere in the archives.
>>
>> I am interested in building a T-SPIM system and ran across an archived conversation on this from June 2014. There was mention that such a setup had been built as well as a possible 3D model of the imaging chamber being available. I couldn't find any additional information on the OpenSPIM.org site and just wanted to ask if there was a link to this information somewhere. Based on the 3D model on the site it looks like you really just need to replicate one arm of the laser path and add a 50/50 splitter near the laser. Would there be needed changes to the operating software? If so, that is beyond my (rather limited) capabilities. However, if instructions exist that'd be great!
>>
>> I appreciate any information you may have on this.
>>
>> Best,
>> David
>> _______________________________________________
>> OpenSPIM mailing list
>> OpenSPIM at openspim.org
>> http://openspim.org/mailman/listinfo/openspim
> _____________
>
> Michael Weber
> Postdoc, Huisken lab
> Max Planck Institute of Molecular Cell Biology and Genetics
> Pfotenhauerstrasse 108, 01307 Dresden
> Tel. 0049 351/2102837
>
> http://www.mpi-cbg.de/huisken
>
> -------------- next part --------------
> An HTML attachment was scrubbed...
> URL: <http://openspim.org/pipermail/openspim/attachments/20150220/82181c4a/attachment-0001.html>
>
> ------------------------------
>
> Message: 2
> Date: Fri, 20 Feb 2015 08:58:50 -0600
> From: David Burk <davidburk1972 at gmail.com>
> To: Michael Weber <weber at mpi-cbg.de>, openspim at openspim.org
> Subject: Re: [OpenSPIM] Information on T-SPIM construction
> Message-ID:
> 	<CAHmt5Uc+6TBr3oBjXWfRu0SVVGXCfCs2x3Gek-0NVjjB=J53Hw at mail.gmail.com>
> Content-Type: text/plain; charset="utf-8"
>
> Thanks, Michael.
>
> Depending on the sample, this might not be ideal, as you get an overlay of
>> "sharp" and "blurry" images.
> Is this due to the fact that one light sheet, say from the left, may not
> have its minimum beam waist at the same position as that from that of the
> right sheet? Or more due to scattering, absorption, etc. of the light as it
> interacts with the specimen?
> I found some images that showed what you are talking about. I just thought
> there may be some 'middle ground' you could settle on by adjusting the
> sheets from either side that would give relatively uniform illumination
> (but with a loss of resolution).
> It does sound like - if you choose the slow manual method - this could be
> doable. Illuminate from left > capture images > close left shutter >
> illuminate from right > capture > merge?
> Perhaps detailed designs of a T-SPIM system will eventually be uploaded to
> the web along with the modifications to the software to drive the
> additional hardware.
>
> Have a great weekend,
> David
>
> On Fri, Feb 20, 2015 at 6:40 AM, Michael Weber <weber at mpi-cbg.de> wrote:
>
>> Hey David,
>>
>> Pete might have a step file for the 3-objective chamber. And yes, in
>> principle you just need two arms and a 50/50 splitter, but there are some
>> limitations. Unless you add shutters or AOTFs, you simultaneously
>> illuminate from both sides. Depending on the sample, this might not be
>> ideal, as you get an overlay of "sharp" and "blurry" images. This typically
>> gets worse with larger samples. You can add manual shutters to be able to
>> choose the side of illumination. If you want to be able to quickly record
>> images with sequential double-sided illumination, you need to integrate
>> shutters/AOTFs into the software.
>>
>> Best,
>> Michael
>>
>> On Feb 18, 2015, at 8:41 PM, David Burk <davidburk1972 at gmail.com> wrote:
>>
>> I apologize in advance if the answer to my question is somewhere in the
>> archives.
>>
>> I am interested in building a T-SPIM system and ran across an archived
>> conversation on this from June 2014. There was mention that such a setup
>> had been built as well as a possible 3D model of the imaging chamber being
>> available. I couldn't find any additional information on the OpenSPIM.org
>> site and just wanted to ask if there was a link to this information
>> somewhere. Based on the 3D model on the site it looks like you really just
>> need to replicate one arm of the laser path and add a 50/50 splitter near
>> the laser. Would there be needed changes to the operating software? If so,
>> that is beyond my (rather limited) capabilities. However, if instructions
>> exist that'd be great!
>>
>> I appreciate any information you may have on this.
>>
>> Best,
>> David
>> _______________________________________________
>> OpenSPIM mailing list
>> OpenSPIM at openspim.org
>> http://openspim.org/mailman/listinfo/openspim
>>
>>
>> _____________
>>
>> Michael Weber
>> Postdoc, Huisken lab
>> Max Planck Institute of Molecular Cell Biology and Genetics
>> Pfotenhauerstrasse 108, 01307 Dresden
>> Tel. 0049 351/2102837
>>
>> http://www.mpi-cbg.de/huisken
>>
>>
> -------------- next part --------------
> An HTML attachment was scrubbed...
> URL: <http://openspim.org/pipermail/openspim/attachments/20150220/30186416/attachment-0001.html>
>
> ------------------------------
>
> _______________________________________________
> OpenSPIM mailing list
> OpenSPIM at openspim.org
> http://openspim.org/mailman/listinfo/openspim
>
>
> End of OpenSPIM Digest, Vol 22, Issue 13
> ****************************************

-- 
Dr. Carsten Wolff
Humboldt University Berlin
Department of Biology / Comparative Zoology
Philippstr. 13, Haus 2
10115 Berlin
Germany
Phone: +49 (0)30 2093 6284