[OpenSPIM] Picard hi-res stage

Johannes Schindelin johannes.schindelin at gmx.de
Mon Feb 16 05:59:09 CST 2015


Hi Monika,

On 2015-02-16 12:02, Monika Pawłowska wrote:
> W dniu .02.2015 o 12:33 Johannes Schindelin 
> <johannes.schindelin at gmx.de>  pisze:
> 
>> The Picard stage driver in Micro-Manager is the only part of the  
>> OpenSPIM software we managed to contribute so far. Therefore: no, it  
>> does not need to be changed.
> 
> the reason I am asking about the Picard stage driver is the following:
> 
> the OpenSPIM plugin should make it possible to move the stage up to 9
> mm.  However, when I set the step size 0.25 um and max number of steps
> 45000 in  the Hardware Configuration, I can only go up to 8191.75 um,
> or 32767  steps. This happens both in the OpenSPIM plugin and when
> using the  MicroManager "Stage control" plugin (see attachement).

This looks like an arbitrary limit, however, I could only see an 
arbitrary limit of 8000 in the source code: 
https://github.com/openspim/micromanager/blob/5f1c8d2079aa2d354820b82faae57e6ce53047d0/DeviceAdapters/PicardStage/PicardStage.cpp#L716

> I use the mFiji  version provided by Ulrik.

I have not the faintest idea from what source code Ulrik has built this 
version – I am still waiting for his Pull Request(s)... ;-)

> Using the software provided by Picard I can go up to 45000 steps.
> 
> Any ideas why this is so?

As I said, I think there are some very arbitrary limits in the source 
code which should be turned into properties that can be set in the 
hardware configuration wizard (with sensible default values, of 
course!), also indicated by the `TODO` I linked above.

Ciao,
Johannes




More information about the OpenSPIM mailing list