[OpenSPIM] Choice of emission filters

[Brau]

Dear members of the openspim mailing list,

Many thanks for all the answers I received. I have a 488/10 and we will 
definitely try this.
Best regards,
Frédéric

Le 19/11/2014 16:20, Hartmann Harz a écrit :
> Dear Frédéric,
> in our open spim setup we had the same issue with the 488 laser like you. We detected laser light in the emission path. The solution - as already mentioned by Pete - is a clean up filter. We found a relative cheap filter from Edmund optics Part No.#65-086. This filter has only a blocking efficiency of 10^-4. However, the filter works fine in our GFP application. This filter fits nicely into the aperture in front of the slit, so there in no need for an additional holder.
>
> Best regards
>
> Hartmann
> -- 
> Dr. H. Harz
> Ludwig-Maximilians-Universität München
> Biozentrum
> AG Prof. Leonhardt
> Raum B02.059
> Großhaderner Straße 2
> 82152 Martinsried
> Germany
> Phone: +49(89)2180-74 259
> Fax: +49(89)218099-74 236
> Mail:harz at biz.uni-muenchen.de


-- 
------------------------------------------------------------------------------------------
Frédéric Brau
Institut de Pharmacologie Moléculaire et Cellulaire - UMR7275 CNRS/UNS
660, Route des Lucioles
06560 VALBONNE
Courriel : brau at ipmc.cnrs.fr
Web : http://www.ipmc.cnrs.fr/
Tel: 33 (0)4 93 95 77 83 Bureau / 77 87 / 77 88 / 77 89 Confocaux
Fax: 33 (0)4 93 95 77 08
Plateforme Microscopie Imagerie Côte d'Azur : http://www.mica-bio.fr
Réseau Technologique de Microscopie de Fluorescence Multidimensionnelle : http://rtmfm.cnrs.fr/
-----------------------------------------------------------------------------------------------

-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://openspim.org/pipermail/openspim/attachments/20141119/3873d42e/attachment-0002.html>