[OpenSPIM] A question about the Light Sheet software

[Luke Stuyvenberg]

Hello Cristian and Alexis,

Please pardon the long delay in my reply!

On 3/24/2014 3:15 PM, Cristian Undurraga wrote:
> Thanks Dr. Alexis for the full answer. I`m tempted to updates the 
> softwares, but I`ll wait for the correct version, :)
>
> We are using the MicroManager version "1.4.x dev", this is a version 
> for "developers"?, it would be better if we use the "users" version?, 
> i.e. 1.4.15 (this have a update for the Cobolt laser made by Dr. 
> Maizel) or the 1.4.14. In these case, I`ll need to configure all the 
> hardware from zero, as shown on the OpenSpim web page 
> (http://openspim.org/How_to_install).
> Best,
>
> Cristian
>
> El 21-03-2014, a las 429, Alexis Maizel escribió:
>
>> Hi Cristian,
>> sorry for the delay.
>>
>> First: do NOT upgrade anything. This will break the ability to 
>> control the laser. (short explanation: to get the Cobolt laser to 
>> work with OpenSPIM I had to essentially rewrite the code of the laser 
>> driver; unfortunately, in between the nice folks at OpenSPIM have 
>> decided to revamp the way OpenSPIM talks to the laser for the new 
>> version of OpenSPIM; hence, if you update you loose the ability to 
>> operate the laser…). I have to hack the code of the current Cobolt 
>> driver to make it compatible with hte latest (and hopefully future) 
>> versions of OpenSPIM. What I need is time...
Alexis -- this is definitely something we should coordinate on; I feel 
we might be missing each other in trying to simultaneously accommodate 
each other. ;-) When it's convenient, feel free to contact me and the 
OpenSPIM list to sort this out; we can set up a long-term solution.

>>>>> We are using the "version 1.4.x dev" with the configuration file 
>>>>> to startup "OpenSPIM01.app.cfg", and the problem is that when we 
>>>>> specify an "Output directory" (in ex. a new folder in the 
>>>>> desktop), the program don`t take the picture or don´t start the 
>>>>> timelapse, but when left empty the "Output directory", we can take 
>>>>> the picture or the TL begins. The default option (with an empty 
>>>>> output directory) is limited for the memory allocated in Fiji 
>>>>> (1024 in RAM), and therefore is limited the acquisition file to 
>>>>> this memory amount.
Christian -- I don't know exactly why this is happening... Could you 
please try to start a time lapse with the output directory specified 
again, then attach the most recent Micro-Manager core log (a file named 
CoreLog########.txt, in the OpenSPIM.app directory)? This will help me 
find the source of the problem so I can fix it. Thanks!

Once again, I apologize for the delay!
Luke Stuyvenberg