[OpenSPIM] Problems with OpenSPIM system

[Mark Tsuchida]

Hi Ke,

On Wed, Jun 25, 2014 at 10:27:54PM -0400, Ke Li wrote:
> I'm Ke, the graduate student asked you questions related to the USB 4D
> stage of Picard Industry about three weeks ago. Thanks for answering my
> questions before. Now I encounter some problems.
> 
> 1. I still can't add the Z stage when I create the hardware configuration.
> I tried both 64-bit and 32-bit Windows system, neither can work. Since the
> problem report doesn't give much info, I'm not sure whether there are other
> ways to solve this problem? And what possible reasons can cause the
> problem?

Are you sure you have the correct serial number for the Z stage (as
Johannes suggested)? In the problem report you sent us, it looked like
you had not set the serial number when adding the Z stage in the
Hardware Configuration Wizard. This causes the device adapter to use the
serial number "-1", which always results in an error.

Do you see a field to enter the serial number for the Z stage? If you cannot
get it to work even when entering the correct serial number, could you try
sending another Problem Report?

Here is the relevant section of the log you sent:
2014-06-25T12:01:05.715866 p:5756 t:3840 [LOG] Device Picard Twister loaded from PicardStage and labeled as Picard Twister
2014-06-25T12:01:05.715866 p:5756 t:3840 [LOG] Device Picard Twister message: Pinging motors...
2014-06-25T12:01:06.191893 p:5756 t:3840 [LOG] Device Picard Twister message: Found 0 motors and 1 twisters.
2014-06-25T12:01:31.622348 p:5756 t:3840 [dbg] Will set property: "Picard Twister"-"Max" = "58980"
2014-06-25T12:01:31.622348 p:5756 t:3840 [dbg] Did set property: "Picard Twister"-"Max" = "58980"
2014-06-25T12:01:31.622348 p:5756 t:3840 [dbg] Will set property: "Picard Twister"-"Min" = "-58980"
2014-06-25T12:01:31.622348 p:5756 t:3840 [dbg] Did set property: "Picard Twister"-"Min" = "-58980"
2014-06-25T12:01:31.622348 p:5756 t:3840 [dbg] Will set property: "Picard Twister"-"Serial Number" = "095"
2014-06-25T12:01:31.651350 p:5756 t:3840 [dbg] Did set property: "Picard Twister"-"Serial Number" = "095"
2014-06-25T12:01:31.651350 p:5756 t:3840 [dbg] Will set property: "Picard Twister"-"StepSize" = "1.8000"
2014-06-25T12:01:31.651350 p:5756 t:3840 [dbg] Did set property: "Picard Twister"-"StepSize" = "1.8000"
2014-06-25T12:01:31.683351 p:5756 t:3840 [LOG] Device Picard Twister initialized.
2014-06-25T12:01:35.499570 p:5756 t:3840 [LOG] Device Picard Z Stage loaded from PicardStage and labeled as Picard Z Stage
2014-06-25T12:01:35.502570 p:5756 t:3840 [LOG] Device Picard Z Stage message: Could not initialize motor -1 (PiUsb error code 1)
2014-06-25T12:01:40.667865 p:5756 t:3840 [LOG] Device Picard XY Stage loaded from PicardStage and labeled as Picard XY Stage
2014-06-25T12:01:51.324475 p:5756 t:3840 [dbg] Will set property: "Picard XY Stage"-"Serial Number (X)" = "274"
2014-06-25T12:01:51.327475 p:5756 t:3840 [dbg] Did set property: "Picard XY Stage"-"Serial Number (X)" = "274"
2014-06-25T12:01:51.327475 p:5756 t:3840 [dbg] Will set property: "Picard XY Stage"-"Serial Number (Y)" = "2751"
2014-06-25T12:01:51.361477 p:5756 t:3840 [LOG] Device Picard XY Stage message: Could not initialize motor 2751 (PiUsb error code 1)
2014-06-25T12:01:51.361477 p:5756 t:3840 [LOG] Error occurred. Device Picard XY Stage. Error in device Picard XY Stage: Unable to communicate with the device.. (Error code: 35)
2014-06-25T12:01:53.600605 p:5756 t:3840 [LOG] default xyStage unloaded.
2014-06-25T12:01:53.600605 p:5756 t:3840 [LOG] Device Picard XY Stage loaded from PicardStage and labeled as Picard XY Stage
2014-06-25T12:01:57.468826 p:5756 t:3840 [dbg] Will set property: "Picard XY Stage"-"Serial Number (X)" = "274"
2014-06-25T12:01:57.471826 p:5756 t:3840 [dbg] Did set property: "Picard XY Stage"-"Serial Number (X)" = "274"
2014-06-25T12:01:57.471826 p:5756 t:3840 [dbg] Will set property: "Picard XY Stage"-"Serial Number (Y)" = "275"
2014-06-25T12:01:57.531830 p:5756 t:3840 [dbg] Did set property: "Picard XY Stage"-"Serial Number (Y)" = "275"
2014-06-25T12:01:57.531830 p:5756 t:3840 [dbg] Will set property: "Picard XY Stage"-"X-Max" = "7800"
2014-06-25T12:01:57.531830 p:5756 t:3840 [dbg] Did set property: "Picard XY Stage"-"X-Max" = "7800"
2014-06-25T12:01:57.531830 p:5756 t:3840 [dbg] Will set property: "Picard XY Stage"-"X-Min" = "0"
2014-06-25T12:01:57.531830 p:5756 t:3840 [dbg] Did set property: "Picard XY Stage"-"X-Min" = "0"
2014-06-25T12:01:57.531830 p:5756 t:3840 [dbg] Will set property: "Picard XY Stage"-"X-StepSize" = "1.5000"
2014-06-25T12:01:57.531830 p:5756 t:3840 [dbg] Did set property: "Picard XY Stage"-"X-StepSize" = "1.5000"
2014-06-25T12:01:57.531830 p:5756 t:3840 [dbg] Will set property: "Picard XY Stage"-"Y-Max" = "7800"
2014-06-25T12:01:57.531830 p:5756 t:3840 [dbg] Did set property: "Picard XY Stage"-"Y-Max" = "7800"
2014-06-25T12:01:57.531830 p:5756 t:3840 [dbg] Will set property: "Picard XY Stage"-"Y-Min" = "0"
2014-06-25T12:01:57.531830 p:5756 t:3840 [dbg] Did set property: "Picard XY Stage"-"Y-Min" = "0"
2014-06-25T12:01:57.531830 p:5756 t:3840 [dbg] Will set property: "Picard XY Stage"-"Y-StepSize" = "1.5000"
2014-06-25T12:01:57.531830 p:5756 t:3840 [dbg] Did set property: "Picard XY Stage"-"Y-StepSize" = "1.5000"
2014-06-25T12:01:57.595834 p:5756 t:3840 [LOG] Device Picard XY Stage initialized.

> 2. As for the steps of installing the OpenSPIM/Fiji package, I can't find
> the *Micro-Manager* or *Micro-Manager studio* when I click the plugins in
> the Micro-Manager (*Plugins->Micro-Manager->Micro-Manager Studio)* I'm
> using in my windows 7 64-bit system, which is the newest version.
> [image: Inline image 1]
> 
> 3. And is the windows 7 64-bit system a potential problem for hardware
> control since Window XP 32-bit is the required system? Can the Windows 7
> 64-bit system be the reason that I can't add the Z stage (But the XY stage
> works well and I tried to add the stage in the 32-bit XP system, the Z
> stage still can't be added)?

I'll let the OpenSPIM folks address these questions. For the Picard
stage specifically, there is no theoretical reason why it shouldn't work
on Windows 7 64 bit, but we have not tested the stage here.

Best,
Mark

-- 
Mark Tsuchida
Micro-Manager Team (UCSF Vale Lab)