[OpenSPIM] T-spim

[Michael Redd]

For those who have built a T-SPIM, I have a several questions. The first question is about creating the second illumination light sheet.  The T-SPIM drawing in the OPENSPIM gallery, a mirror is placed near the laser that splits the light between two identical light paths each complete with cylindrical lenses etc.. My question is rather than creating two identical light paths with many optical components, why can't a the light sheet be created in a single light path and a splitting mirror be placed at the end prior to the 10x Olympus dipping lens and routed with mirrors to the back of the other 10x dipping lens.  The second question concerns shuttering the laser. Does anyone have any  shutters that they would recommend for use with a T-SPIM? Finally, does anyone have a digital plans for a T-SPIM sample chamber?

thanks

Michael Redd