[OpenSPIM] Lightsheet characterization

[Michael Weber]

Hey Jan,

I started a page about light sheet characterization in the wiki, maybe you want to contribute to it?

http://openspim.org/Light_sheet_characterization

Best,
Michael

On Apr 6, 2014, at 3:34 PM, "Krieger, Jan" <j.krieger at Dkfz-Heidelberg.de> wrote:

> Dear Julien!
> 
> you should use a mirror mounted under 45°. This is described in detail in http://www.freidok.uni-freiburg.de/volltexte/1533/pdf/huisken_phd.pdf, or e.g. in http://staff.science.nus.edu.sg/~chmwt/publications/Singh%20AP%202013%202D%20array_supplementary.pdf and http://www.dkfz.de/Macromol/publications/files/spimfccs2013_supplement.pdf
> 
> Then you can do a Gaussian fit to cuts through the light sheet.
> 
> Best,
> JAN
> 
> 
> Dipl.-Phys. Jan Krieger
> German Cancer Research Center (dkfz)
> Department B040 - Biophysics of Macromolecules (Prof. J. Langowski)
> Im Neuenheimer Feld 580
> 69120 Heidelberg
> 
> fon: +49 / 6221 / 42-3395
> fax: +49 / 6221 / 42-3391
> e-mail: j.krieger at dkfz.de
> www: http://www.dkfz.de/Macromol/
> ________________________________________
> Von: openspim-bounces at openspim.org [openspim-bounces at openspim.org] im Auftrag von Julien DUMONT [julien.dumont at inaf.cnrs-gif.fr]
> Gesendet: Sonntag, 6. April 2014 14:59
> An: openspim at openspim.org
> Betreff: [OpenSPIM] Lightsheet characterization
> 
> Hi,
> 
> On our setup, we would like to measure precisely the width of the lightsheet at the focal point, but I'm running out of ideas to do that, as the resolution of our beam profiler is not able to go down to the size of it. Is anyone of you have a trick to do that ?
> 
> Cheers
> 
> Julien Dumont
> --
> Ingénieur d'étude microscopie
> 1 Avenue de la Terrasse
> CNRS, NeD, Bâtiment 32
> 91190 Gif-sur-Yvette
> 01.69.82.34.19
> 
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_____________

Michael Weber
PhD Student, Huisken lab
Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstrasse 108, 01307 Dresden
Tel. 0049 351/2102837

http://www.mpi-cbg.de/huisken

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