[OpenSPIM] Lightsheet characterization

[Krieger, Jan]

Dear Julien!

you should use a mirror mounted under 45°. This is described in detail in http://www.freidok.uni-freiburg.de/volltexte/1533/pdf/huisken_phd.pdf, or e.g. in http://staff.science.nus.edu.sg/~chmwt/publications/Singh%20AP%202013%202D%20array_supplementary.pdf and http://www.dkfz.de/Macromol/publications/files/spimfccs2013_supplement.pdf

Then you can do a Gaussian fit to cuts through the light sheet.

Best,
JAN


Dipl.-Phys. Jan Krieger
German Cancer Research Center (dkfz)
Department B040 - Biophysics of Macromolecules (Prof. J. Langowski)
Im Neuenheimer Feld 580
69120 Heidelberg

fon: +49 / 6221 / 42-3395
fax: +49 / 6221 / 42-3391
e-mail: j.krieger at dkfz.de
www: http://www.dkfz.de/Macromol/
________________________________________
Von: openspim-bounces at openspim.org [openspim-bounces at openspim.org] im Auftrag von Julien DUMONT [julien.dumont at inaf.cnrs-gif.fr]
Gesendet: Sonntag, 6. April 2014 14:59
An: openspim at openspim.org
Betreff: [OpenSPIM] Lightsheet characterization

Hi,

On our setup, we would like to measure precisely the width of the lightsheet at the focal point, but I'm running out of ideas to do that, as the resolution of our beam profiler is not able to go down to the size of it. Is anyone of you have a trick to do that ?

Cheers

Julien Dumont
--
Ingénieur d'étude microscopie
1 Avenue de la Terrasse
CNRS, NeD, Bâtiment 32
91190 Gif-sur-Yvette
01.69.82.34.19

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