[OpenSPIM] WG: Antwort: Re: "Stack at this Z plus" problem persisting

edgar.escobar.nieto at ipt.fraunhofer.de edgar.escobar.nieto at ipt.fraunhofer.de
Thu Oct 31 12:26:29 CDT 2013 

Hi Luke,

About the error files, you are right, they appear as long as I close  µManager/Fiji. The "Stack at this Z plus" button is the only problem, I  can
still made everthing else as I menctioned before. I just updated Fiji and the problem persist. The button is unstucked as long as I move the mouse
cursor outside the area of the button and I am able to click it again. The problem is comparable as if I place the cursor over a normal button e.g.
Oh Snap!, then click the mouse and move the cursor outside Oh Snap! and finally release the mouse outside Oh Snap! 
If you do that simply nothing happens. That is just the way the "Stack at this Z plus" button behaves.

I also thought that the problem was related with the TIS cam but I already test Fiji in a different computer (Windows 7 64 bits). In that computer
is not installed any driver or software from the camera. I made a test using only the Picard Stage, a demo shutter and a demo camera
and the problem about "Stack at this Z plus" remains, the only difference I noticed is that when I close µManager/Fiji there is no error files.

Hope this information is useful. Have a happy longweekend. 

Kind regards,
Edgar




 _________________________________________________________________________

Fraunhofer-Institut für Produktionstechnologie IPT 
Edgar Escobar Nieto  
 

 
 
Steinbachstraße 17 
52074 Aachen 

edgar.escobar.nieto at ipt.fraunhofer.de 
http://www.ipt.fraunhofer.de
_________________________________________________________________________
 
 
 


-----Luke Stuyvenberg <stuyvenberg at wisc.edu> schrieb: -----
An: edgar.escobar.nieto at ipt.fraunhofer.de
Von: Luke Stuyvenberg <stuyvenberg at wisc.edu>
Datum: 30.10.2013 20:54
Kopie: openspim at openspim.org
Betreff: Re: WG: Antwort: Re: "Stack at this Z plus" problem persisting

Hi Edgar,

Unfortunately the files were not as revealing as I had hoped. They seem to indicate a persistent problem with either the TIScam device adapter or the way we interact with it. (Most of the hardware errors point into TIScam's extra DLL -- TIS_DShowLib10.dll+0x2eced.) I will eventually need to do some digging to try and discern the source of that error; however, I'm not convinced it's the cause of the stack insert problem (another symptom of the problem, perhaps) -- if the software isn't crashing when you click the button, then these files are probably being generated when Micro-Manager is closed.


When you click the 'insert stack' button, it is only that button that sticks, correct? The remaining buttons still work? You can change between tabs, move motors, et cetera? Can it be un-stuck and clicked on again? It's still possible that there is a silent exception being generated; I'll check on that.


Also, please try updating to the latest version again -- I recently pushed an update that should eliminate the need for the demo-shutter trick by being prepared for the absence of a laser.


Thanks,
Luke

On 10/28/13, edgar.escobar.nieto at ipt.fraunhofer.de wrote:
> Hi Luke,
> 
> I attach the files that you requested, I hope they can give you a clue about the problem.
> 
> Thank you and kind regards,
> Edgar
> 
> 
> _________________________________________________________________________
> 
> Fraunhofer-Institut für Produktionstechnologie IPT 
> Edgar Escobar Nieto 
> 
> 
> 
> 
> Steinbachstraße 17 
> 52074 Aachen 
> 
> edgar.escobar.nieto at ipt.fraunhofer.de 
> http://www.ipt.fraunhofer.de 
> _________________________________________________________________________ 
> 
> 
> 
> 
> 
> 
> -----Weitergeleitet von Edgar Escobar Nieto/Fraunhofer IPT am 28.10.2013 11:28 ----- An: Luke Stuyvenberg <stuyvenberg at wisc.edu>
> Von: Edgar Escobar Nieto/Fraunhofer IPT
> Datum: 25.10.2013 21:40
> Kopie: openspim at openspim.org
> Betreff: Antwort: Re: "Stack at this Z plus" problem persisting
> 
> Hi Luke,
> 
> I will send you all the information requested on monday, since now I am not at the
> institute. Hopefully with the no laser patch the problem could be solved, but we have
> to try first. 
> 
> About the "Stack at this Z plus" button, it is the only problem, it looks that it sticks. I can still
> move the stage, change the tabs, add a new single position in the position list, use snap
> images and save them in RAM or directly in disk. In resume, I can do everything except set a 
> stack of positions in the position list.
> 
> Thanks to you!! and see you on monday.
> 
> Kind regards,
> Edgar
> 
> 
> _________________________________________________________________________
> 
> Fraunhofer-Institut für Produktionstechnologie IPT 
> Edgar Escobar Nieto 
> 
> 
> 
> 
> Steinbachstraße 17 
> 52074 Aachen 
> 
> edgar.escobar.nieto at ipt.fraunhofer.de 
> http://www.ipt.fraunhofer.de 
> _________________________________________________________________________ 
> 
> 
> 
> 
> 
> 
> -----Luke Stuyvenberg <stuyvenberg at wisc.edu> schrieb: ----- An: edgar.escobar.nieto at ipt.fraunhofer.de
> Von: Luke Stuyvenberg <stuyvenberg at wisc.edu>
> Datum: 25.10.2013 20:14
> Kopie: openspim at openspim.org
> Betreff: Re: "Stack at this Z plus" problem persisting
> 
> Hi Edgar,
> 
> 
> Please don't worry about disturbing me; it's what I am here for, after all!
> 
> 
> You are absolutely correct about the missing TIScam DLLs; I forgot to include them in the new version. I will head to LOCI and correct that in two hours or so.
> 
> 
> I'm still at a loss with regards to the errors you are encountering, however. I will have a patch for the 'no laser' problem early next week, hopefully alongside the autostart fix, but until we can diagnose the acquisition bug, I won't know how to fix it.
> 
> 
> With that in mind, I have some more information to request:
> - Please attach to your reply all core logs, hs error files, and configuration files (your active one, in particular), compressed with your file archiver of choice.
> - Please list a) all hardware involved in the setup (with model numbers, if you have them), and b) all devices you set up in the hardware configuration.
> - When you click the 'insert stack' button, you say it sticks down and nothing happens -- is the rest of the UI still responsive? Can you change tabs? Move the stage?
> 
> 
> I'm casting a pretty wide net, here, but hopefully somewhere in there will be a vital clue.
> 
> 
> Thanks,
> Luke
> 
> 
> 
> edgar.escobar.nieto at ipt.fraunhofer.de wrote:
> 
> Dear Luke,
> 
> I am sorry to disturb you again but I have been struggling a lot with SPIM acquisiton plugin :(. Hopefully I made some discoveries.
> 
> I updated Fiji and again had the problem again about the TIScam (The Imaging Source camera). But I discover that 
> mmgr_dal_TIScam.dll depends on TIS_DShowLib10.dll and TIS_UDSHL10.dll but in the mm\Win32 location are
> TIS_UDSHL09_vc9d.dll and TIS_DShowLib09.dll. I got the version 10 of the DLLs from the 1.14.16 version of a plain 
> installation of MicroManager and just copied them to mm\Win32. Maybe you can solve that from the Fiji updater: to uninstall 
> the version 9 of the DLLs and to install the version 10. Anayway the problem about the camera is fixed now. I attach the
> version 10 of the DLLs to this mail.
> 
> About the "Stack at this Z plus" button I still have the problem when using the updated version of Fiji. I also discover that if I don't 
> use the demo shuter I can't write the images direclty to disk, the message "Error acquiring: null" appears and in the output directory is saved a .ome.tiff
> empty image (0 byte). So for the moment I have to use the no updated version of Fiji in order to perform SPIM acquisiton.
> 
> The problem about using the no updated version of Fiji is that the plugin don't respond when pressing "Oh Snap" (as
> it happens when pressing "Stack at this Z plus" in the Fiji updated version) when I want to write the images directly to disk.
> 
> So at the end if I want to perform SPIM image acquisition (the way I have done that until now), I have to use the no updated version 
> of Fiji and save the images to RAM no specifying an ouput directory. The problem is that I am limited by the RAM and all the images
> are saved in the same stack even if different positions are specified in the position list.
> 
> Finally to be sure that the problem about the "Stack at this Z plus" button is not related to the computer I am using (Windows XP 32 bit)
> I tried Fiji in a different computer (Windows 7 64 bit).
> 
> I really would like to use the SPIM image acquisition plugin and benefit from all his capabilities.
> 
> Thanks in advance for your comments.
> 
> Kind regards,
> Edgar
> 
> 
> 
> 
> 
> 
> 
> _________________________________________________________________________
> 
> Fraunhofer-Institut fr Produktionstechnologie IPT 
> Edgar Escobar Nieto 
> 
> 
> 
> 
> Steinbachstraáe 17 
> 52074 Aachen 
> 
> edgar.escobar.nieto at ipt.fraunhofer.de 
> http://www.ipt.fraunhofer.deÿ
> _________________________________________________________________________
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