[OpenSPIM] WG: Antwort: Re: "Stack at this Z plus" problem persisting

Mon Oct 28 05:31:53 CDT 2013

 Hi Luke,

I attach the files that you requested, I hope they can give you a clue about the problem.

Thank you and kind regards,
Edgar
 _________________________________________________________________________

Fraunhofer-Institut für Produktionstechnologie IPT 
Edgar Escobar Nieto  

Steinbachstraße 17 
52074 Aachen 

edgar.escobar.nieto at ipt.fraunhofer.de 
http://www.ipt.fraunhofer.de
_________________________________________________________________________

-----Weitergeleitet von Edgar Escobar Nieto/Fraunhofer IPT am 28.10.2013 11:28 -----
An: Luke Stuyvenberg <stuyvenberg at wisc.edu>
Von: Edgar Escobar Nieto/Fraunhofer IPT
Datum: 25.10.2013 21:40
Kopie: openspim at openspim.org
Betreff: Antwort: Re: "Stack at this Z plus" problem persisting

 Hi Luke,

I will send you all the information requested on monday, since now I am not at the
institute. Hopefully with the no laser patch the problem could be solved, but we have
to try first. 

About the "Stack at this Z plus" button, it is the only problem, it looks that it sticks. I can still
move the stage, change the tabs, add a new single position in the position list, use snap
images and save them in RAM or directly in disk. In resume, I can do everything except set a 
stack of positions in the position list.

Thanks to you!! and see you on monday.

Kind regards,
Edgar
 _________________________________________________________________________

Fraunhofer-Institut für Produktionstechnologie IPT 
Edgar Escobar Nieto  

Steinbachstraße 17 
52074 Aachen 

edgar.escobar.nieto at ipt.fraunhofer.de 
http://www.ipt.fraunhofer.de
_________________________________________________________________________

-----Luke Stuyvenberg <stuyvenberg at wisc.edu> schrieb: -----
An: edgar.escobar.nieto at ipt.fraunhofer.de
Von: Luke Stuyvenberg <stuyvenberg at wisc.edu>
Datum: 25.10.2013 20:14
Kopie: openspim at openspim.org
Betreff: Re: "Stack at this Z plus" problem persisting

Hi Edgar,

Please don't worry about disturbing me; it's what I am here for, after all!

You are absolutely correct about the missing TIScam DLLs; I forgot to include them in the new version. I will head to LOCI and correct that in two hours or so.

I'm still at a loss with regards to the errors you are encountering, however. I will have a patch for the 'no laser' problem early next week, hopefully alongside the autostart fix, but until we can diagnose the acquisition bug, I won't know how to fix it.

With that in mind, I have some more information to request:
- Please attach to your reply all core logs, hs error files, and configuration files (your active one, in particular), compressed with your file archiver of choice.
- Please list a) all hardware involved in the setup (with model numbers, if you have them), and b) all devices you set up in the hardware configuration.
- When you click the 'insert stack' button, you say it sticks down and nothing happens -- is the rest of the UI still responsive? Can you change tabs? Move the stage?

I'm casting a pretty wide net, here, but hopefully somewhere in there will be a vital clue.

Thanks,
Luke

edgar.escobar.nieto at ipt.fraunhofer.de wrote:

 Dear Luke,

I am sorry to disturb you again but I have been struggling a lot with SPIM acquisiton plugin :(. Hopefully I made some discoveries.

I updated Fiji and again had the problem again about the TIScam (The Imaging Source camera). But I discover that 
 mmgr_dal_TIScam.dll depends on TIS_DShowLib10.dll and  TIS_UDSHL10.dll but in the mm\Win32 location are
TIS_UDSHL09_vc9d.dll  and TIS_DShowLib09.dll. I got the version 10 of the DLLs from the 1.14.16 version of a plain 
 installation of MicroManager and just copied them to  mm\Win32. Maybe you can solve that from the Fiji updater: to uninstall 
 the version 9 of the DLLs and to install the version 10. Anayway the problem about the camera is fixed now. I attach the
 version 10 of the DLLs to this mail.

About the "Stack at this Z plus" button I still have the problem when using the updated version of Fiji. I also discover that if I don't 
 use the demo shuter I can't write the images direclty to disk, the message "Error acquiring: null" appears and in the output directory is saved a .ome.tiff
 empty image (0 byte). So for the moment I have to use the no updated version of Fiji in order to perform SPIM acquisiton.

The problem about using the no updated version of Fiji is that the plugin don't respond when pressing "Oh Snap" (as
 it happens when pressing "Stack at this Z plus" in the Fiji updated version) when I want to write the images directly to disk.

So at the end if I want to perform SPIM image acquisition (the way I have done that until now), I have to use the no updated version 
 of Fiji and save the images to RAM no specifying an ouput directory. The problem is that I am limited by the RAM and all the images
 are saved in the same stack even if different positions are specified in the position list.

Finally to be sure that the problem about the "Stack at this Z plus" button is not related to the computer I am using (Windows XP 32 bit)
 I tried Fiji in a different computer (Windows 7 64 bit).

I really would like to use the SPIM image acquisition plugin and benefit from all his capabilities.

Thanks in advance for your comments.

Kind regards,
Edgar

  _________________________________________________________________________

Fraunhofer-Institut für Produktionstechnologie IPT 
Edgar Escobar Nieto  

Steinbachstraáe 17 
52074 Aachen 

edgar.escobar.nieto at ipt.fraunhofer.de  
 http://www.ipt.fraunhofer.de 
_________________________________________________________________________ 

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