[OpenSPIM] "Stack at this Z plus" problem persisting

[Luke Stuyvenberg]

Hi Edgar,

Please don't worry about disturbing me; it's what I am here for, after all!

You are absolutely correct about the missing TIScam DLLs; I forgot to include them in the new version. I will head to LOCI and correct that in two hours or so.

I'm still at a loss with regards to the errors you are encountering, however. I will have a patch for the 'no laser' problem early next week, hopefully alongside the autostart fix, but until we can diagnose the acquisition bug, I won't know how to fix it.

With that in mind, I have some more information to request:
- Please attach to your reply all core logs, hs error files, and configuration files (your active one, in particular), compressed with your file archiver of choice.
- Please list a) all hardware involved in the setup (with model numbers, if you have them), and b) all devices you set up in the hardware configuration.
- When you click the 'insert stack' button, you say it sticks down and nothing happens -- is the rest of the UI still responsive? Can you change tabs? Move the stage?

I'm casting a pretty wide net, here, but hopefully somewhere in there will be a vital clue.

Thanks,
Luke

edgar.escobar.nieto at ipt.fraunhofer.de wrote:

>Dear Luke,
>
>I am sorry to disturb you again but I have been struggling a lot with SPIM acquisiton plugin :(. Hopefully I made some discoveries.
>
>I updated Fiji and again had the problem again about the TIScam (The Imaging Source camera). But I discover that 
>mmgr_dal_TIScam.dll depends on TIS_DShowLib10.dll and TIS_UDSHL10.dll but in the mm\Win32 location are
>TIS_UDSHL09_vc9d.dll and TIS_DShowLib09.dll. I got the version 10 of the DLLs from the 1.14.16 version of a plain 
>installation of MicroManager and just copied them to mm\Win32. Maybe you can solve that from the Fiji updater: to uninstall 
>the version 9 of the DLLs and to install the version 10. Anayway the problem about the camera is fixed now. I attach the
>version 10 of the DLLs to this mail.
>
>About the "Stack at this Z plus" button I still have the problem when using the updated version of Fiji. I also discover that if I don't 
>use the demo shuter I can't write the images direclty to disk, the message "Error acquiring: null" appears and in the output directory is saved a .ome.tiff
>empty image (0 byte). So for the moment I have to use the no updated version of Fiji in order to perform SPIM acquisiton.
>
>The problem about using the no updated version of Fiji is that the plugin don't respond when pressing "Oh Snap" (as
>it happens when pressing "Stack at this Z plus" in the Fiji updated version) when I want to write the images directly to disk.
>
>So at the end if I want to perform SPIM image acquisition (the way I have done that until now), I have to use the no updated version 
>of Fiji and save the images to RAM no specifying an ouput directory. The problem is that I am limited by the RAM and all the images
>are saved in the same stack even if different positions are specified in the position list.
>
>Finally to be sure that the problem about the "Stack at this Z plus" button is not related to the computer I am using (Windows XP 32 bit)
>I tried Fiji in a different computer (Windows 7 64 bit).
>
>I really would like to use the SPIM image acquisition plugin and benefit from all his capabilities.
>
>Thanks in advance for your comments.
>
>Kind regards,
>Edgar
>
>
>
>
>
>_________________________________________________________________________
>
>Fraunhofer-Institut für Produktionstechnologie IPT 
>Edgar Escobar Nieto 
>
>
>
>
>Steinbachstraße 17 
>52074 Aachen 
>
>edgar.escobar.nieto at ipt.fraunhofer.de 
>http://www.ipt.fraunhofer.de 
>_________________________________________________________________________ 
>
>
>
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