[OpenSPIM] Zebra fish SPIM imaging

[Pavel Tomancak]

Dear Edgar,

I am not sure I understand what you did. How did you stitch them together? Is this multi-view (angle) data or a tiling across a large field of view?

In any case you most likely do not have enough beads in there. I would say you need ten times more.

Your beads are gigantic, 2um, we use much smaller beads typically.

Let me know more details and I am sure we can work it out.

All the best

PAvel

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Pavel Tomancak, Ph.D.

Research Group Leader
Max Planck Institute of Molecular Cell Biology and Genetics in Dresden
Pfotenhauerstr. 108
D-01307 Dresden							Tel.: +49 351 210 2670
Germany									Fax: +49 351 210 2020

tomancak at mpi-cbg.de
http://www.mpi-cbg.de/research/research-groups/pavel-tomancak.html
twitter: @PavelTomancak
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On Oct 24, 2013, at 5:38 PM, edgar.escobar.nieto at ipt.fraunhofer.de wrote:

> Hi dear All,
> 
> I have made some imaging of a zebra fish that expresses GFP fluorescence with a heat shock, I have gotten 25 stacks (5x5) and stitched it together.
> 2 µm green beads were embedded  to the agarose and I think that the beads signal is good in contrast to the signal of the fish. 
> 
> I would like to know if with the concentration of beads that could be appreciated in the Z projection and the video of the scanning of the resulting stack
> is enough to perform multiview fusion.
> 
> https://www.dropbox.com/s/sooicfk68iszq3m/Fused_linear%20blending_18fps.avi
> https://www.dropbox.com/s/q98akotaynltbvc/Fused_linear%20blending_z%20projection.tif
> 
> Thanks in advance for your comments.
> 
> Kind regards,
> Edgar
> _________________________________________________________________________
> 
> Fraunhofer-Institut für Produktionstechnologie IPT 
> Edgar Escobar Nieto 
> 
> 
> 
> 
> Steinbachstraße 17 
> 52074 Aachen 
> 
> edgar.escobar.nieto at ipt.fraunhofer.de 
> http://www.ipt.fraunhofer.de 
> _________________________________________________________________________ 
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