[OpenSPIM] SPIM with no laser control

[Aurelia Honerkamp-Smith]

Hi everyone,

I guess now is a good time to mention that I built my SPIM using a laser
line we already had instead of purchasing the Coherent Cube, by diverting
the existing line to the SPIM with a flip mirror and a periscope.  Laser
power control is manual. The fact that I have no laser connected is causing
a problem for SPIM acquisition, though.  I get an error message that my
setup is invalid when I first start the plugin; then, although acquisition
through the Video tab works OK, I can't acquire a regular stack or
timelapse: I get "Error acquiring: null" when I push the Oh Snap button.  I
need to change the plugin so that it ignores the laser power control, and
uses a Thorlabs shutter for blinking instead of the laser.  I realize this
might be a tall order, so I'm ready to start working on it myself, but I
would appreciate being pointed in the right direction for how to do this
most efficiently.

Thanks again,
Aurelia



On Fri, Sep 27, 2013 at 12:03 PM, Aurelia Honerkamp-Smith <
aureliaomega at gmail.com> wrote:

> Perfect, the 26 September nightly build version of the PVCAM adapter has
> cleared up my live view problem!  Thanks very much, I am so happy!
> Aurelia
>
>
>
> On Fri, Sep 27, 2013 at 4:15 AM, Luke Stuyvenberg <stuyvenberg at wisc.edu>wrote:
>
>> Hi Aurelia,
>>
>>
>> On Thu, 26 Sep 2013, Aurelia Honerkamp-Smith wrote:
>>
>>  Thanks for this latest update: it has almost fixed my PVCAM problem.
>>> Now I can load the camera, and since the last update I no longer get a
>>> time-out error message when I use the live view.  But, the live window
>>> opens and displays an image, then it doesn't refresh, so I see the same
>>> image until I stop and start live view again.  I can now run the Open
>>> SPIM plugin, but have to keep stopping and starting live view in order
>>> to see what is going on with my calibration sample.  I'm so close!  Any
>>> suggestions for fixing this?  Live view through my camera works fine in
>>> a plain, non-Fiji and non-OpenSPIM installation of Micromanager, so I
>>> can align and take pictures at the moment, but I want to use your lovely
>>> plugin.
>>>
>> Yes, I seem to remember other reports of this trouble (also with PVCAM,
>> if I recall correctly), though I had thought them resolved... I believe
>> Johannes is correct, and synchronizing with Micro-Manager may resolve this
>> issue. I've started work on this, but the process is (frustratingly)
>> intricate, and might take some time. In the future I hope to make the
>> process unnecessary.
>>
>> As a temporary measure, you should have no trouble using the PVCAM device
>> adapter from the Micro-Manager nightly build (The file should be wherever
>> you installed the 'vanilla' Micro-Manager to; simply copy it to OpenSPIM's
>> "mm/win32" directory -- it may ask you to overwrite). If you decide to try
>> this, please let me know how it works out.
>>
>> Thanks,
>> Luke
>>
>
>
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