[OpenSPIM] Some early images

[Johannes Schindelin]

Hi Luke,

On Fri, 14 Jun 2013, Luke Stuyvenberg wrote:

> On 06/14/13, Johannes Schindelin wrote:
> 
> > Also, as I told Julie on her way out: we absolutely have to use
> > off-frequency beads. Our signal is too delicate to be overlaid by
> > beads having a field day.
> 
> I agree. Before we finished imaging, Julie decided to work with the red
> beads on Monday; next Thursday we should be able to put together some
> samples with weaker beads.
>  
> > This might need some changes on the Wiki to make it *very clear* that
> > you should *never* use beads that are excited by exactly the
> > wavelength of the laser.
> 
> I should point out that these are 20um beads causing the flares. It's
> possible that sub-resolution beads (even those specifically excited by
> our laser) won't have the same dramatic effect as these. In other words
> '*never*' might be a bit overzealous; I'm sure there are times when
> operators would like a very strong bead signal.

Given that Dresden had substantial problems even with sub-resolution
beads, I would actually wager a bet that "never" is the correct adjective
here: I highly doubt that any non-toxic amounts of fluorophores translated
from reporter genes would come even close to the amount of fluorophores
present even in the tiniest sub-resolution beads made by humans.

In any case, the idea of OpenSPIM is to make things easier by sharing
knowledge, right? So even if I'd lose my bet, it would be the appropriate
thing to share the insight that non-off-color beads *were* too bright in
our case.

Ciao,
Johannes