[OpenSPIM] Open SPIM

[Kevin W Eliceiri]

its the one listed in the link to Edmunds Optics on that webpage, the direct link is http://www.edmundoptics.com/testing-targets/test-targets/resolution-test-targets/opal-glass-ronchi-ruling-slides/2838

It looks like Edmunds Website is having problems at the moment, so you could also call them to order, its part# 58607, MULTI-GRID STANDARD MICROMETER

To make it fit, we scored with a diamond pencil as shown in the picture. You could also other similar micrometer products sold by Thorlabs etc.

best
k


On 06/10/13, "LIM Soon Yew John (IMB)"  wrote:
> Dear Johannes and Peter,
> 
> Thank you vey much for the information. Can I please find out the part number for the mirror grid used in the light sheet calibration as this information is not included in http://openspim.org/Light-sheet_Calibration.
> 
> Best Regards,
> John
> ________________________________________
> From: Johannes Schindelin [Johannes.Schindelin at gmx.de]
> Sent: Tuesday, June 04, 2013 11:15 PM
> To: Peter Gabriel Pitrone
> Cc: LIM Soon Yew John (IMB); Srivats HARIHARAN (IMB); openspim at openspim.org
> Subject: Re: [OpenSPIM] Open SPIM
> 
> Dear John,
> 
> On Tue, 4 Jun 2013, Peter Gabriel Pitrone wrote:
> 
> > The lightsheet with the stated optics is around 7-10 um. We are working
> > on a lightsheet thickness calculator that we will put on the wiki as
> > soon as it it finished.
> 
> To enhance on that comment until the time when Dr Jan chimes in, this is
> how we measure the light-sheet thickness: we use the same mirror as for
> the light-sheet calibration described here:
> 
> http://openspim.org/Light-sheet_Calibration
> 
> (unfortunately, the description of the last steps of that page have not
> yet been completed.)
> 
> In the last steps, what we do is to use an improvised transmission light
> (we really use a reading light and shine that through the sample chamber)
> to get the mirror into focus both with the transmission light and the
> light-sheet by having the mirror (also called grid on the page because it
> is a gridded mirror) at 45 degrees such that the light-sheet is reflected
> directly into the camera.
> 
> At that stage, the light-sheet thickness can be measured exactly by
> calibrating the pixel spacing with the known grid spacing and the simply
> counting the number of pixels of the width (because the light-sheet is now
> directed directly into the camera, parallel to the detection axis).
> 
> The height of the light-sheet is configurable to a certain extent: you can
> make it narrower or larger by adjusting the iris in the last part of the
> illumination axis. Of course, you still want it to be relatively uniform
> over the height; we found it reasonable uniform in the field of view we
> use (and we made it a little larger than that). Although I have to admit
> that we never came around to *really* quantify this.
> 
> Ciao,
> Johannes
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--
Kevin W. Eliceiri
Director, Laboratory for Optical and Computational Instrumentation (LOCI)
Departments Cell and Molecular Biology and Biomedical Engineering
Affiliate Principal Investigator, Morgridge Institute for Research (MIR)
Room 271 Animal Sciences, 1675 Observatory Drive, Madison, WI 53706
Phone: 608-263-6288