[OpenSPIM] Lasers for SPIM

[Fredrik Ek]

Hi,

we are setting up a SPIM system based on the openspim configuration with some additional features (Orca Flash 4, 3 lasers and emission filter Wheel). We are in the process of purchasing lasers and I have some questions:

1. What is your opinion when it comes to the power of lasermodules. I noted that Jan Krieger in a recent mail pointed out the DPSS lasers could be flucturating once below 10% of their nominal power. We are going to use them for analysis of zebrafish larvae and what is a typical power setting for running these kind of experiments.

2. Fiberconnected or open beam. Your opinions.

3. Beamcombiners (light hub Sole or similar) or seperate lasers? Other than Jan that have similar experiences of problems with alignment.

4. AOTF module or not?

Your input is highly appreciated. 

Thanks!


Fredrik Ek, 
Researcher, Lund University Sweden