Personal tools


From OpenSPIM

Revision as of 09:48, 1 February 2013 by Weber (talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to: navigation, search

The flexibility of light sheet microscopes such as OpenSPIM allows for different configurations. The standard setup consists of one illumination arm ("1I") and one detection arm ("1D"). One can add a second illumination arm ("2I") to improve the homogeneity of the light sheet illumination and/or to reduce the need for rotation. A second detection arm ("2D") enables the simultaneous acquisition from both sides.

basic configurations

1I 1D OpenSPIM 03.jpg 1I 1D single-sided illumination
single-sided detection
xxx / xxx mm (w/d)
2I 1D OpenSPIM 02.jpg 2I 1D double-sided illumination
single-sided detection
xxx / xxx mm (w/d)
2I 2D OpenSPIM 02.jpg 2I 2D double-sided illumination
double-sided detection
xxx / xxx mm (w/d)

high-throughput / multi-sample configurations

2I 1D OpenSPIM farm 02.jpg FARM double-sided illumination
single-sided detection
stack of 4 setups
xxx / xxx / xxx mm (w/d/h)