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Table of parts 1-1

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All OpenSPIM designed parts need to be double checked for tolerances if you will be 3D printing them!! Having a well trained machinist is an invaluable resource, we recommend that you purchase all of your parts first and then have your OpenSPIM designed parts made to fit afterwards by someone with experience!!

Illumination Parts List

Manufacturer Accessibility Description File or Link/Model # Image Quantity Price (EUR)
Coherent purchase Whichever wavelength & mW is necessary for your experiments Cube
Coherent CUBE.jpg
1 ~7000
OpenSPIM self made Functions as heat sink and brings the laser to the desired height of 50 mm off the surface of the optical breadboard

Must be made out of a heat conducting material!! We use Aluminum.
.stl
.step
.pdf
OpenSPIM Cube-Laser Heatsink.JPG
1
Thorlabs purchase Ø1/2"/12.7 mm SM05-Mounted Frosted Glass Alignment Disk w/Ø1 mm Hole

For use in aligning the illumination beam path
DG05-1500-H1-MD
ThorLabs DG05-1500-H1-MD Alignment-Disk.jpg
1 25
ThorLabs purchase Ø1"/25.4 mm AR-Coated Absorptive Neutral Density Filter, SM1-Threaded Mount, AR coating 350-700 nm

For use in diminishing the intensity of the illumination source
NE10A-A (10%)
NE13A-A (5%)
NE20A-A (1%)
NE30A-A (0.1%)
ThorLabs NE05A-A ND-Filter.jpg
1 60
ThorLabs
Semrock
Chroma
Omega
optional purchase

although highly recommended for any diode laser
Ø1"/25.4 mm Fluorescent Microscopy Filter

For use with laser clean up
Make sure to get a bandpass with a narrow FWHM around the wavelength of the illumination. (for example: 488/10)
AHF Fluorescent-Filter.jpg
xx 40
ThorLabs purchase Ø1"/25.4 mm Flip Mount - Metric

For use in manipulating the lightsheet: such as inserting a neutral density or cleanup filter into illumination beam path
TRF90/M
ThorLabs TRF90-M Filter-Flipper.jpg
2 70

Opto-mechanical parts list

Manufacturer Accessibility Description File or Link/Model # Image Quantity Price (EUR)
ThorLabs purchase The metric (M6 tapped 25 mm spacing) optical bread board that the OpenSPIM system is installed on MB3045/M
ThorLabs MB3045-M Aluminum-Breadboard 300x450x12-7mm.jpg
1 185
ThorLabs purchase Metric Dovetail Optical Rail, 300 mm

(One will be modified)
RLA300/M
ThorLabs RLA075-M Meteric-Dovetail-Optical-Rail Length=300mm.jpg
2 65
ThorLabs purchase Metric Dovetail Optical Rail, 150 mm

(At least one will be modified)
RLA150/M
ThorLabs RLA075-M Meteric-Dovetail-Optical-Rail Length=150mm.jpg
2 35
ThorLabs purchase Metric Dovetail Optical Rail, 75 mm RLA075/M
ThorLabs RLA075-M Meteric-Dovetail-Optical-Rail Length=75mm.jpg
1 25
ThorLabs purchase Rail carrier 1"/25.4 mm

(some will need to be modified)
RC1
ThorLabs RC1-M Rail-Carrier for Dovetail-Optical-Rail.jpg
11 20
OpenSPIM self made elevates the iris 8.85 mm from the modified rail carrier to the desired height of 50 mm off the surface of the optical breadboard .stl
.step
.pdf
OpenSPIM Stilt Iris.JPG
1 xx
ThorLabs purchase Ø1"/25.4 mm optic mount (for the iris) LMR1/M
ThorLabs LMR1-M Lens-Mount Dia=1in.jpg
1 15
ThorLabs purchase Ring-Activated SM1 Iris Diaphragm SM1D12D
ThorLabs SM1D12D Ring-Activated-SM1-Iris-Diaphragm.jpg
1 55
OpenSPIM self made elevates the vertical slit 13.17 mm from the rail carrier to the desired height of 50 mm off the surface of the optical breadboard .stl
.step
.pdf
OpenSPIM Stilt Vertical-slit.JPG
1 xx
ThorLabs purchase Adjustable mechanical slit VA100/M
ThorLabs VA100-M Adjustable-Mechanical-Slit Metric.jpg
1 220
OpenSPIM self made elevates the Ø1/2"/12.7 mm lenses 14.95 mm from the rail carrier to the desired height of 50 mm off the surface of the optical breadboard .stl
.step
.pdf
OpenSPIM Stilt 1-2in-Lens.JPG
5 xx
ThorLabs purchase Ø1/2"/12.7 mm optic mount (for light sheet creation) LMR05/M
ThorLabs LMR05-M Lens-Mount Dia=0-5in.jpg
7 15
OpenSPIM self made elevates the Ø1/2"/12.7 mm mirror mounts 22.57 mm from the rail carrier to the desired height of 50 mm off the surface of the optical breadboard .stl
.step
.pdf
OpenSPIM Stilt 1-2in-Mirror.JPG
2 xx
ThorLabs purchase Kinematic Mount for Ø1/2"/12.7 mm Optics, Metric KM05/M
ThorLabs KM05-M.jpg
2 35
OpenSPIM self made elevates the Ø1"/25.4 mm mirror mount 24.55 mm to the desired height of 50 mm off the surface of the optical breadboard .stl
.step
.pdf
OpenSPIM Stilt 1in-Mirror.JPG
1 xx
ThorLabs purchase Ø1"/25.4 mm Gimbal Mirror Mount, Metric GM100/M
ThorLabs GM100-M.jpg
1 200
ThorLabs purchase Metric Rotation Mount for Ø1"/25.4 mm optics, 360° Continuous or 15° Indexed Rotation RSP1X15/M
ThorLabs RSP1X15-M Metric Rotation-Mount Dia=1in.jpg
1 110

Illumination Axis Optical Components List

Manufacturer Accessibility Description File or Link/Model # Image Quantity Price (EUR)
ThorLabs purchase Ø1" broadband dielectric mirror BB1-E02
ThorLabs BB1-E02 Broadband-Dielectric-Mirror Diameter=1in.jpg
1 65
ThorLabs purchase Ø1/2" broadband dielectric mirror BB05-E02
ThorLabs BB05-E02 Broadband-Dielectric-Mirror Diameter=0-5in.jpg
2 45
ThorLabs purchase Ø1/2" Achromatic Doublet, SM05-Threaded Mount, f=50 mm, ARC: 400-700 nm AC127-050-A-ML
ThorLabs AC127-050-A-ML Achromatic-Doublet Dia=0-5in F=50mm.jpg
2 65
ThorLabs purchase Ø1/2" Achromatic Doublet, SM05-Threaded Mount, f=25 mm, ARC: 400-700 nm AC127-025-A-ML
ThorLabs AC127-025-A-ML Achromatic-Doublet Dia=0-5in F=25mm.jpg
2 65
ThorLabs purchase f = 50 mm, Ø1" Cylindrical Achromat, AR Coating: 350 - 700 nm ACY254-050-A
ThorLabs ACY254-050-A Cylindrical-Achromat Dia=1in f=50mm.jpg
1 315
Olympus purchase Water dipping lens for Illumination (10x/0.3) UMPLFLN 10XW
Olympus UMPLFLN-10XW 10x-Water-Dippining-Objective-Lens.jpg
1 ~700

SPIM Chamber Parts List

Manufacturer Accessibility Description File or Link/Model # Image Quantity Price (EUR)
OpenSPIM self made holds the two Olympus water dipping objectives in a perpendicular arrangement to illuminate and observe the sample Sample Chamber.stl
Sample Chamber.step
Sample Chamber.pdf

Chamber Holder.stl
Chamber Holder.step
Chamber Holder.pdf
OpenSPIM SPIM Chamber-Holder.JPG
1 xx
Lelebeck.de purchase NBR 70 20x3 mm O-Ring - Creates a water tight seal around the Olympus water dipping objectives 1164
O-ring.jpg
2 0.10

Detection Axis Optical Components List

Manufacturer Accessibility Description File or Link/Model # Image Quantity Price (EUR)
Olympus purchase Water dipping objective lens for detection (20x/0.5) UMPLFLN 20XW
Olympus UMPLFLN-20XW 20x-Water-Dippining-Objective-Lens.jpg
1 ~900
OpenSPIM self made The detection axis holder holds everything behind the detection objective to the camera at the desired height of 50 mm off the surface of the optical breadboard. Base.stl
Base.step
Base.pdf

Top.stl
Top.step
Top.pdf
OpenSPIM Detection-Axis-Holder.JPG
1 xx
OpenSPIM self made The infinity space defines the distance between the detection objective and it's tube lens. .stl
.step
.pdf
OpenSPIM Infinity-Space.JPG
1 xx
ThorLabs
Semrock
Chroma
Omega
purchase Ø1"/25.4 mm Fluorescent Microscopy Filter

get a few for different experiments
Make sure to get at least one good bandpass (FWHM 30-50 nm) and a longpass that block the illumination well
AHF Fluorescent-Filter.jpg
xx 100-300
OpenSPIM make as many as you need Ø1"/Ø25.4 mm microscopy fluorescence emission filter holder that fits into OpenSPIM's infinity space

Here are some companies who make Ø1"/Ø25.4 mm microscopy filters:
Semrock, Chroma,
Omega, and even ThorLabs.
Base.stl
Base.step
Base.pdf

Top.stl
Top.step
Top.pdf
OpenSPIM 1in Micro Filter-Holder Assembly.JPG
xx
OpenSPIM make as many as you need

If you need them...
Ø1 1/4"/Ø31.5 mm astronomy filter holder that fits into OpenSPIM's infinity space Base.stl
Base.step
Base.pdf

Top.stl
Top.step
Top.pdf
OpenSPIM 1-25in Astro Filter-Holder Assembly.JPG
 ? xx
Olympus purchase Tube lens for detection axis U-TLU
021 diffView.jpg
1 ~400
Olypmus purchase the one you need 0.5x magnifying camera mount U-TV0.5XC-3 xx 1 ~400
Olympus purchase the one you need 1x magnifying camera mount U-TV1 X & U-CMAD3 xx 1 ~400

4D Motor System Parts List

Manufacturer Accessibility Description File or Link/Model # Image Quantity Price (EUR)
Picard-Industries optional purchase 4D positioning of the sample USB-4D-Stage
4D-Stage.jpg
1 Ask for quote

Optional Parts List

Manufacturer Accessibility Description File or Link/Model # Image Quantity Price (EUR)
ThorLabs optional purchase M4 Cap Screw and Hardware Kit HW-KIT1/M
ThorLabs HW-KIT2-M M6-Capscrew-set.jpg
1 50
ThorLabs optional purchase M6 Cap Screw and Hardware Kit HW-KIT2/M
ThorLabs HW-KIT1-M M4-Capscrew-set.jpg
1 95
ThorLabs optional purchase SM1 Spanner Wrench SPW602 or SPW606
ThorLabs SPW606.jpg
1 25