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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">Dear Alessandro,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">I can recommend to have a look at these publications from Frederic Strobl in Ernst Stelzer’s Group. The mDSLM also has a stage holding
the specimen from below. Frederic established some clever mounting techniques for Tribolium.
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US"><a href="https://www.jove.com/video/55629/light-sheet-based-fluorescence-microscopy-living-or-fixed-stained">https://www.jove.com/video/55629/light-sheet-based-fluorescence-microscopy-living-or-fixed-stained</a><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US"><a href="https://www.nature.com/articles/nprot.2017.028">https://www.nature.com/articles/nprot.2017.028</a><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">I am a plant biologist and roots need oxygen too. I thought about emptying the chamber in between time points or move the sample outside
to let the tissue get in contact with air but to be honest I have never tried it. Instead I use a perfusion system and stir or bubble air in the medium outside of the microscope chamber, which helps!<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">Greetings!<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">Daniel<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
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<p class="MsoNormal" style="border:none;padding:0cm"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Daniel von Wangenheim<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">Plant & Crop Sciences<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">School of Biosciences<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">University of Nottingham<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">Sutton Bonington Campus<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US">LE12 5RD<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
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<p class="MsoNormal"><b><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif">From:</span></b><span lang="EN-US" style="font-size:11.0pt;font-family:"Calibri",sans-serif"> OpenSPIM <openspim-bounces@openspim.org>
<b>On Behalf Of </b>Alessandro Ciccarelli<br>
<b>Sent:</b> 12 April 2018 11:03<br>
<b>To:</b> openspim@openspim.org<br>
<b>Subject:</b> [OpenSPIM] Imaging drosophila pupae<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
<p><span style="font-family:"Calibri",sans-serif;color:black">Dear light-sheet users,<o:p></o:p></span></p>
<p><span style="font-family:"Calibri",sans-serif;color:black">does anybody have experience in long-term imaging of drosophila pupae with light-sheet? I`m currently looking for suggestions about how to mount this tricky sample; actually the pupa needs to be:<o:p></o:p></span></p>
<p><span style="font-family:"Calibri",sans-serif;color:black">- partially in contact with air (it needs to breath)<o:p></o:p></span></p>
<p><span style="font-family:"Calibri",sans-serif;color:black">- immersed in water (I`m using water dipping lenses)<o:p></o:p></span></p>
<p><span style="font-family:"Calibri",sans-serif;color:black"><o:p> </o:p></span></p>
<p><span style="font-family:"Calibri",sans-serif;color:black">I`m currently using a Luxendo MuVi-SPIM, where the holder is coming from the bottom of the chamber.
<o:p></o:p></span></p>
<p><span style="font-family:"Calibri",sans-serif;color:black">Thanks in advance!<o:p></o:p></span></p>
<p><span style="font-family:"Calibri",sans-serif;color:black">Best,<o:p></o:p></span></p>
<p><span style="font-family:"Calibri",sans-serif;color:black">Alessandro<o:p></o:p></span></p>
<p><span style="font-family:"Calibri",sans-serif;color:black"><o:p> </o:p></span></p>
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<p class="paragraph" style="vertical-align:baseline"><span class="normaltextrun"><span lang="EN-US" style="color:#7F7F7F">---</span></span><span class="eop"><span lang="EN-US"> </span></span><span lang="EN-US" style="font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
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<p class="paragraph" style="vertical-align:baseline"><span class="normaltextrun"><span lang="EN-US" style="color:#7F7F7F">Alessandro Ciccarelli </span></span><span class="eop"><span lang="EN-US"> </span></span><span lang="EN-US" style="font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
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<p class="paragraph" style="vertical-align:baseline"><span class="normaltextrun"><i><span lang="EN-US" style="color:#7F7F7F">Light Sheet Specialist</span></i></span><span class="eop"><span lang="EN-US"> </span></span><span lang="EN-US" style="font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
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<p class="paragraph" style="vertical-align:baseline"><span class="normaltextrun"><span lang="EN-US" style="color:#7F7F7F">CALM - Crick Advanced Light Microscopy Facility</span></span><span class="eop"><span lang="EN-US"> </span></span><span lang="EN-US" style="font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
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<p class="paragraph" style="vertical-align:baseline"><span class="normaltextrun"><span lang="EN-US" style="color:#7F7F7F">The Francis Crick Institute</span></span><span class="eop"><span lang="EN-US"> </span></span><span lang="EN-US" style="font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
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<p class="paragraph" style="vertical-align:baseline"><span class="normaltextrun"><span lang="EN-US" style="color:#7F7F7F">1 Midland Road - London -NW1 1AT</span></span><span class="eop"><span lang="EN-US"> </span></span><span lang="EN-US" style="font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
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<p class="paragraph" style="vertical-align:baseline"><span class="normaltextrun"><span lang="EN-US" style="color:#7F7F7F">United Kingdom</span></span><span class="eop"><span lang="EN-US"> </span></span><span lang="EN-US" style="font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
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<p class="paragraph" style="vertical-align:baseline"><span class="eop"><span lang="EN-US"> </span></span><span lang="EN-US" style="font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
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<p class="paragraph" style="vertical-align:baseline"><span class="normaltextrun"><span lang="EN-US" style="color:#7F7F7F">T: +442037960913</span></span><span class="eop"><span lang="EN-US"> </span></span><span lang="EN-US" style="font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
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<p class="paragraph" style="vertical-align:baseline"><span class="normaltextrun"><span lang="EN-US" style="color:#7F7F7F">E: </span></span><span lang="EN-US" style="font-family:"Calibri",sans-serif"><a href="mailto:alessandro.ciccarelli@crick.ac.uk" target="_blank" title="mailto:alessandro.ciccarelli@crick.ac.uk Ctrl+Click or tap to follow the link" id="LPNoLP"><span class="normaltextrun"><span style="font-family:"Times New Roman",serif;color:#7F7F7F">alessandro.ciccarelli@crick.ac.uk</span></span></a></span><span class="eop"><span lang="EN-US" style="font-size:11.0pt"> </span></span><span lang="EN-US" style="font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
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<p class="paragraph" style="vertical-align:baseline"><span class="normaltextrun"><span lang="EN-US" style="color:#7F7F7F">W: </span></span><span lang="EN-US" style="font-family:"Calibri",sans-serif"><a href="http://www.crick.ac.uk/" target="_blank" id="LPNoLP"><span class="normaltextrun"><span style="font-family:"Times New Roman",serif;color:#7F7F7F">www.crick.ac.uk</span></span></a></span><span class="eop"><span lang="EN-US" style="font-size:11.0pt;font-family:"Arial",sans-serif"> </span></span><span lang="EN-US" style="font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">From:</span></b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black"> OpenSPIM <<a href="mailto:openspim-bounces@openspim.org">openspim-bounces@openspim.org</a>>
on behalf of Jan Huisken <<a href="mailto:huisken@mpi-cbg.de">huisken@mpi-cbg.de</a>><br>
<b>Sent:</b> 09 April 2018 21:52<br>
<b>To:</b> Feinstein, Timothy N; <a href="mailto:openspim@openspim.org">openspim@openspim.org</a><br>
<b>Subject:</b> Re: [OpenSPIM] holographic diffusers</span><span style="font-family:"Calibri",sans-serif;color:black">
<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black"> <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black">Hi Tim
<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black">We have not yet tried those diffusers.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black">Why is your mSPIM not working? Sounds like your resonant mirror is not in the right plane. Please let us know if there is anything we can help with.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black">Best<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black">Jan<o:p></o:p></span></p>
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<p class="MsoNormal" style="margin-bottom:12.0pt"><span style="font-family:"Calibri",sans-serif;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black">On Apr 9, 2018, at 8:50 AM, Feinstein, Timothy N <<a href="mailto:tnf8@PITT.EDU" id="LPlnk578761">tnf8@PITT.EDU</a>> wrote:<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Calibri",sans-serif;color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">Hi folks, </span><span style="font-family:"Calibri",sans-serif;color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black"> </span><span style="font-family:"Calibri",sans-serif;color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">Salili and co-workers (Review Sci. Instruments 89: 036107) just published a very interesting technique for eliminating stripe artifacts using elliptical holographic
diffusers. It looks like a much simpler version of mSPIM, with just a couple filters and no moving parts. This would be great news for me since my mSPIM design doesn’t work – instead of tilting, the sheet moves up and down in a parallel track. </span><span style="font-family:"Calibri",sans-serif;color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black"> </span><span style="font-family:"Calibri",sans-serif;color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">Has anyone tried holographic diffusers before? They look like they need to be quite close to the sample, so it’s possible they would not work with a conventional
openSPIM sample holder. Any feedback would be appreciated. </span><span style="font-family:"Calibri",sans-serif;color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black"> </span><span style="font-family:"Calibri",sans-serif;color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">Thanks!</span><span style="font-family:"Calibri",sans-serif;color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black"> </span><span style="font-family:"Calibri",sans-serif;color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black"><br>
Tim</span><span style="font-family:"Calibri",sans-serif;color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black"> </span><span style="font-family:"Calibri",sans-serif;color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri",sans-serif;color:black">Timothy Feinstein, Ph.D. </span><span style="font-family:"Calibri",sans-serif;color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri",sans-serif;color:black">Research Scientist</span><span style="font-family:"Calibri",sans-serif;color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri",sans-serif;color:black">Department of Developmental Biology</span><span style="font-family:"Calibri",sans-serif;color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri",sans-serif;color:black">University of Pittsburgh</span><span style="font-family:"Calibri",sans-serif;color:black"><o:p></o:p></span></p>
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</span><span style="font-family:"Calibri",sans-serif;color:black"><a href="mailto:OpenSPIM@openspim.org" id="LPlnk662653"><span style="font-size:9.0pt;font-family:"Helvetica",sans-serif;color:#954F72">OpenSPIM@openspim.org</span></a></span><span style="font-size:9.0pt;font-family:"Helvetica",sans-serif;color:black"><br>
</span><span style="font-family:"Calibri",sans-serif;color:black"><a href="http://openspim.org/mailman/listinfo/openspim" id="LPlnk459532"><span style="font-size:9.0pt;font-family:"Helvetica",sans-serif;color:#954F72">http://openspim.org/mailman/listinfo/openspim</span></a><o:p></o:p></span></p>
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<p><i><span style="font-size:10.0pt;font-family:"Trebuchet MS",sans-serif;color:#707173">The Francis Crick Institute Limited is a registered charity in England and Wales no. 1140062 and a company registered in England and Wales no. 06885462, with its registered
office at 1 Midland Road London NW1 1AT<o:p></o:p></span></i></p>
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