<div dir="ltr">Matt,<div><br></div><div>I haven't had a need for CO2 yet so I can't help you there but I have been successfully heating my chamber to 27C for up to 24 hours. Longer wouldn't be a problem. Samples are largely zebrafish. The chamber is anodized aluminum and the liquid volume is 150mL. I cap the chamber to slow evaporation while leaving a hole for the sample mount to move. I went the simple route and attached two polyimide encapsulated copper foils, via PSA, to the chamber bottom paired with a very low tech controller, an NTC thermistor and an old 12V laptop power supply. The foils and controller are from Minco (<a href="https://www.minco.com/Components/Shop-by-Product">https://www.minco.com/Components/Shop-by-Product</a>). Your chamber size will dictate the specific foil but an example would be, say, part number HK6912. The controller I used is the CT325. Throw in a fuse to keep OHS moderately happy. You'll need to calculate the thermal mass of your chamber plus medium (plus anything the chamber touches i.e. transfer heat) and the warm-up time blah blah and match the parts correctly but that's all straightforward. My chamber warms up from 18C to 27C in about 10 minutes. Put the thermistor as close as you can to the sample. I put a temp probe in an agarose mount and adjusted the CT325 to give me 27 at the probe. It's not sexy but it works and it was cheap at ~$200 USD.</div><div><br></div><div>If I wanted CO2 control, I would probably do nothing fancier than a well sealed plexi box around the sample chamber and stage. Feed in heated CO2/air dual gas (or air/N2/CO2 tri-gas) at a slight positive pressure and you don't need CO2 controllers. I would include an IR sensor (not a wet sensor) as it's good form and allow record keeping. Add a gas pressure sensor and wire every sensor into a beagle bone to record all the data. You might find an old system designed for a microscope nobody wants anymore and up-cycle the parts. I would put the stage inside the box as a bigger box is way cheaper and simpler than soft sealing the sample holder arm. Or go crazy and box everything but the camera and lasers with opaque plexi and you get gas, temp and safety all in one. Send me photos if you build one.</div><div><div class="gmail_extra"><br></div><div class="gmail_extra">Good luck.</div><div class="gmail_extra"><br clear="all"><div><div class="gmail_signature"><div dir="ltr"><div><div dir="ltr"><div><span style="font-size:12.8px">- David</span><br></div><div><br></div><div><font size="1">David Potter</font></div><div><font size="1">Lattice Light Sheet Microscopy Manager</font></div><div><font size="1">Monash Micro Imaging - Advanced Optical Microscopy</font></div><div><font size="1">Monash University Clayton, VIC 3800, Australia</font></div><div><br></div><div><br></div></div></div></div></div></div>
<br><div class="gmail_quote">On 30 January 2018 at 05:00, <span dir="ltr"><<a href="mailto:openspim-request@openspim.org" target="_blank">openspim-request@openspim.org</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">Send OpenSPIM mailing list submissions to<br>
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1. Heating/CO2 solutions for sample chamber (PEARSON Matthew)<br>
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Message: 1<br>
Date: Mon, 29 Jan 2018 12:26:04 +0000<br>
From: PEARSON Matthew <<a href="mailto:matthew.pearson@igmm.ed.ac.uk">matthew.pearson@igmm.ed.ac.uk</a><wbr>><br>
To: "<a href="mailto:openspim@openspim.org">openspim@openspim.org</a>" <<a href="mailto:openspim@openspim.org">openspim@openspim.org</a>><br>
Subject: [OpenSPIM] Heating/CO2 solutions for sample chamber<br>
Message-ID: <<a href="mailto:E944BD3E-A8A2-4190-8BBD-B04593DE95D4@exseed.ed.ac.uk">E944BD3E-A8A2-4190-8BBD-<wbr>B04593DE95D4@exseed.ed.ac.uk</a>><br>
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Hi all,<br>
<br>
I was wondering if anyone has any advice/experience they'd like to share on how to implement heating (up to 37C) and 5% CO2 supply to a SPIM sample chamber. What samples do you image and over what kind of time frame?<br>
<br>
Thanks for the help,<br>
<br>
Matt<br>
<br>
--<br>
Matt Pearson<br>
Microscopy Facility<br>
MRC Human Genetics Unit<br>
Institute of Genetics and Molecular Medicine (IGMM)<br>
University of Edinburgh<br>
Crewe Road<br>
EH4 2XU<br>
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