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<p>Hello Kathleen,</p>
<p>attached is an image of the tools that you can use for alignment:
left a homebuild version (without grids), right a professional
version (unfortunately not for sale).</p>
<p>The Edmund Multi-Grid test is based on a standard glass
coverslip; the patterns are a very thin metal coating. You can cut
the glass coverslip with a diamond pen, but getting the small
pieces you need is somewhat tricky. Apparently it works better
when you first make all the cuts needed, then break the coverslip.</p>
<p>The test target can be reused. Ideally, just use distilled water
in your chamber while aligning. If you need to use buffer, inspect
the glue regularly to make sure it does not dissolve (may damage
your objektives if it's in solution and then deposits on them) and
rinse the test target afterwards.</p>
<p>Best, Wiebke<br>
</p>
<pre class="moz-signature" cols="72">--
------------------------------------------------------------
Wiebke Jahr
PhD Student
Tel. +49 351 210-2768
Huisken Lab,
Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauer Str. 108, 01307 Dresden
------------------------------------------------------------</pre>
<div class="moz-cite-prefix">On 31/07/2017 17:22, Kathleen Chen
wrote:<br>
</div>
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cite="mid:CAGv7iOjDuqO-u0CWUrP+pN5ooz6PKor5iTHej1mcYWD2PfPObQ@mail.gmail.com"
type="cite">
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<div dir="ltr">Hello OpenSPIM members,
<div><br>
</div>
<div>I was wondering how other members performed the alignment
of the light sheet. </div>
<div><br>
</div>
<div>The wiki suggests the use of a portion of the Edmund Optics
Multi-Grid test grid glued to a syringe with a small mirror.
However, there are not many details regarding the making of
this test grid/mirror syringe part so I have been having some
trouble with the alignment.</div>
<div><br>
</div>
<div>This method seems to require cutting out the grid from the
EO part, which from the images seems to be metal. Also, can
this grid/mirror syringe setup be reused or do we need to use
a new grid every time the sheet needs to be aligned? Have
others experienced any trouble with this or do you have any
tips/suggestions?</div>
<div><br>
</div>
<div>Thank you,</div>
<div>Kathleen Chen</div>
</div>
<br>
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