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<p><span id="OLK_SRC_BODY_SECTION" style="font-family: Calibri,
sans-serif; font-size: 14px; font-style: normal; font-variant:
normal; font-weight: normal; letter-spacing: normal;
line-height: normal; orphans: auto; text-align: start;
text-indent: 0px; text-transform: none; white-space: normal;
widows: auto; word-spacing: 0px; -webkit-text-stroke-width:
0px;" class="">Dear Jan and Tim,<br>
</span></p>
<p><span id="OLK_SRC_BODY_SECTION" style="font-family: Calibri,
sans-serif; font-size: 14px; font-style: normal; font-variant:
normal; font-weight: normal; letter-spacing: normal;
line-height: normal; orphans: auto; text-align: start;
text-indent: 0px; text-transform: none; white-space: normal;
widows: auto; word-spacing: 0px; -webkit-text-stroke-width:
0px;" class="">Sorry to come into your discussions but I noticed
your comment Jan concerning this point : a) Double-sided
illumination helps. Depending on the sample you may want to do
simultaneous or sequential double-sided illumination. There are
various ways of merging the two images.</span></p>
<p><span id="OLK_SRC_BODY_SECTION" style="font-family: Calibri,
sans-serif; font-size: 14px; font-style: normal; font-variant:
normal; font-weight: normal; letter-spacing: normal;
line-height: normal; orphans: auto; text-align: start;
text-indent: 0px; text-transform: none; white-space: normal;
widows: auto; word-spacing: 0px; -webkit-text-stroke-width:
0px;" class="">In our lab we built a home-made ultramicroscope
(and reproduce an open-spim :
<a class="moz-txt-link-freetext" href="http://unice.fr/plateformes/mica/ressources/developpements-1/developpements">http://unice.fr/plateformes/mica/ressources/developpements-1/developpements</a>)
and I was wondering about the best method to fuse images taken
from the left+those taken from the right from our
ultramicroscope (without any rotation). At the moment we only do
averaging which maybe not the best solution, I suppose. Do you
know a paper mentionning the different ways to do image fusion,
or any advice about that (I also knew about a method consisting
to cut the image in 3 part, but I don't really remember all the
steps)?</span></p>
<p><span id="OLK_SRC_BODY_SECTION" style="font-family: Calibri,
sans-serif; font-size: 14px; font-style: normal; font-variant:
normal; font-weight: normal; letter-spacing: normal;
line-height: normal; orphans: auto; text-align: start;
text-indent: 0px; text-transform: none; white-space: normal;
widows: auto; word-spacing: 0px; -webkit-text-stroke-width:
0px;" class="">Thanks in advance,</span></p>
<p><span id="OLK_SRC_BODY_SECTION" style="font-family: Calibri,
sans-serif; font-size: 14px; font-style: normal; font-variant:
normal; font-weight: normal; letter-spacing: normal;
line-height: normal; orphans: auto; text-align: start;
text-indent: 0px; text-transform: none; white-space: normal;
widows: auto; word-spacing: 0px; -webkit-text-stroke-width:
0px;" class="">Best regards,</span></p>
<p><span id="OLK_SRC_BODY_SECTION" style="font-family: Calibri,
sans-serif; font-size: 14px; font-style: normal; font-variant:
normal; font-weight: normal; letter-spacing: normal;
line-height: normal; orphans: auto; text-align: start;
text-indent: 0px; text-transform: none; white-space: normal;
widows: auto; word-spacing: 0px; -webkit-text-stroke-width:
0px;" class="">Frédéric<br>
</span></p>
<br>
<div class="moz-cite-prefix">Le 05/07/2016 à 20:35, Jan Huisken a
écrit :<br>
</div>
<blockquote
cite="mid:C5F79F6B-AB3E-4176-BD26-F25038B85955@mpi-cbg.de"
type="cite">
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
Hi Tim,
<div class=""><br class="">
</div>
<div class="">great! I like your setup.</div>
<div class=""><br class="">
</div>
<div class="">One remark to the last figure caption in your
document: You need to adjust the amplitude to get the maximum
efficiency of the mSPIM. You start with a small amplitude and
increase it until the light sheet intensity (and fluorescence)
drops. Now you have found the amplitude where you pivot the
sheet as much as the NA of your illumination lens allows and you
are getting the best performance. Going any further, the beam
will be cut off and you loose laser power.</div>
<div class=""><br class="">
</div>
<div class="">Best</div>
<div class="">Jan</div>
<div class=""><br class="">
<div>
<blockquote type="cite" class="">
<div class="">On Jul 5, 2016, at 3:18 PM, Feinstein, Timothy
N <<a moz-do-not-send="true"
href="mailto:tnf8@PITT.EDU" class="">tnf8@PITT.EDU</a>>
wrote:</div>
<br class="Apple-interchange-newline">
<div class="">
<div style="font-family: Calibri, sans-serif; font-size:
14px; font-style: normal; font-variant: normal;
font-weight: normal; letter-spacing: normal;
line-height: normal; orphans: auto; text-align: start;
text-indent: 0px; text-transform: none; white-space:
normal; widows: auto; word-spacing: 0px;
-webkit-text-stroke-width: 0px;" class="">
<div class="">
<div class="">Hi folks, </div>
<div class=""><br class="">
</div>
<div class="">Many thanks for your help. After much
testing I have concluded that anti-striping along
the lines of Huisken & Stainier (2007) is
extremely important for imaging large structures
like a zebrafish embryo. This adds significant
complexity and cost (~$2,000) to the basic openSPIM
design but in my opinion it makes the instrument
dramatically more useful for segmentation and
quantiative imaging. Importantly the fact that I
could make it suggests that it is within the
engineering abilities of most people who can build
an openSPIM. We have not started using it yet as
our lasers are also out for an upgrade; I will
update the list when I have had a chance to test it.
</div>
<div class=""><br class="">
</div>
<div class="">I had to fill in some details from the
2007 paper on my own, so it is likely that more
elegant/less kludgy designs exist. Still, this
guide I wrote for our group may be useful for those
thinking of adding anti-striping in the future. </div>
<div class=""><br class="">
</div>
<div class=""><a moz-do-not-send="true"
href="https://www.dropbox.com/s/6r1z13pggat7v76/Anti-striping%20mirror.docx?dl=0"
style="color: purple; text-decoration: underline;"
class="">https://www.dropbox.com/s/6r1z13pggat7v76/Anti-striping%20mirror.docx?dl=0</a></div>
<div class=""><br class="">
</div>
<div class="">All the best, </div>
<div class=""><br class="">
</div>
<div class=""><br class="">
</div>
<div class="">Tim</div>
<div class=""><br class="">
</div>
<div class="">
<div class="">Timothy Feinstein, Ph.D. </div>
<div class="">Research Scientist</div>
<div class="">University of Pittsburgh Department of
Developmental Biology</div>
<div class=""><br class="">
</div>
</div>
</div>
</div>
<div style="font-family: Calibri, sans-serif; font-size:
14px; font-style: normal; font-variant: normal;
font-weight: normal; letter-spacing: normal;
line-height: normal; orphans: auto; text-align: start;
text-indent: 0px; text-transform: none; white-space:
normal; widows: auto; word-spacing: 0px;
-webkit-text-stroke-width: 0px;" class=""><br class="">
</div>
<span id="OLK_SRC_BODY_SECTION" style="font-family:
Calibri, sans-serif; font-size: 14px; font-style:
normal; font-variant: normal; font-weight: normal;
letter-spacing: normal; line-height: normal; orphans:
auto; text-align: start; text-indent: 0px;
text-transform: none; white-space: normal; widows: auto;
word-spacing: 0px; -webkit-text-stroke-width: 0px;"
class="">
<div style="font-family: Calibri; font-size: 11pt;
text-align: left; border-width: 1pt medium medium;
border-style: solid none none; padding: 3pt 0in 0in;
border-top-color: rgb(181, 196, 223);" class=""><span
style="font-weight: bold;" class="">From:<span
class="Apple-converted-space"> </span></span><<a
moz-do-not-send="true"
href="mailto:openspim-bounces@openspim.org"
style="color: purple; text-decoration: underline;"
class=""><a class="moz-txt-link-abbreviated" href="mailto:openspim-bounces@openspim.org">openspim-bounces@openspim.org</a></a>> on
behalf of Timothy Feinstein <<a
moz-do-not-send="true" href="mailto:tnf8@pitt.edu"
style="color: purple; text-decoration: underline;"
class=""><a class="moz-txt-link-abbreviated" href="mailto:tnf8@pitt.edu">tnf8@pitt.edu</a></a>><br class="">
<span style="font-weight: bold;" class="">Date:<span
class="Apple-converted-space"> </span></span>Tuesday,
May 3, 2016 at 9:27 AM<br class="">
<span style="font-weight: bold;" class="">To:<span
class="Apple-converted-space"> </span></span>Jan
Huisken <<a moz-do-not-send="true"
href="mailto:huisken@mpi-cbg.de" style="color:
purple; text-decoration: underline;" class="">huisken@mpi-cbg.de</a>><br
class="">
<span style="font-weight: bold;" class="">Cc:<span
class="Apple-converted-space"> </span></span>"<a
moz-do-not-send="true"
href="mailto:openspim@openspim.org" style="color:
purple; text-decoration: underline;" class=""><a class="moz-txt-link-abbreviated" href="mailto:openspim@openspim.org">openspim@openspim.org</a></a>"
<<a moz-do-not-send="true"
href="mailto:openspim@openspim.org" style="color:
purple; text-decoration: underline;" class="">openspim@openspim.org</a>><br
class="">
<span style="font-weight: bold;" class="">Subject:<span
class="Apple-converted-space"> </span></span>Re:
[OpenSPIM] Is anyone using digital scanning to reduce
banding?<br class="">
</div>
<div class=""><br class="">
</div>
<div xmlns:v="urn:schemas-microsoft-com:vml"
xmlns:o="urn:schemas-microsoft-com:office:office"
xmlns:w="urn:schemas-microsoft-com:office:word"
xmlns:m="http://schemas.microsoft.com/office/2004/12/omml"
xmlns="http://www.w3.org/TR/REC-html40" class="">
<div link="blue" vlink="purple" class="" lang="EN-US">
<div class="WordSection1" style="page:
WordSection1;">
<div style="margin: 0in 0in 0.0001pt; font-size:
12pt; font-family: 'Times New Roman', serif;"
class=""><span style="font-size: 11pt;
font-family: Calibri, sans-serif; color:
rgb(31, 73, 125);" class="">Correction, I
meant 0.05% tricaine. <o:p class=""></o:p></span></div>
<div style="margin: 0in 0in 0.0001pt; font-size:
12pt; font-family: 'Times New Roman', serif;"
class=""><span style="font-size: 11pt;
font-family: Calibri, sans-serif; color:
rgb(31, 73, 125);" class=""><o:p class=""> </o:p></span></div>
<div style="margin: 0in 0in 0.0001pt; font-size:
12pt; font-family: 'Times New Roman', serif;"
class=""><span style="font-size: 11pt;
font-family: Calibri, sans-serif; color:
rgb(31, 73, 125);" class="">Best,<o:p class=""></o:p></span></div>
<div style="margin: 0in 0in 0.0001pt; font-size:
12pt; font-family: 'Times New Roman', serif;"
class=""><span style="font-size: 11pt;
font-family: Calibri, sans-serif; color:
rgb(31, 73, 125);" class=""><br class="">
<br class="">
T<o:p class=""></o:p></span></div>
<div style="margin: 0in 0in 0.0001pt; font-size:
12pt; font-family: 'Times New Roman', serif;"
class=""><span style="font-size: 11pt;
font-family: Calibri, sans-serif; color:
rgb(31, 73, 125);" class=""><o:p class=""> </o:p></span></div>
<div class="">
<div style="border-style: solid none none;
border-top-color: rgb(181, 196, 223);
border-top-width: 1pt; padding: 3pt 0in 0in;"
class="">
<div style="margin: 0in 0in 0.0001pt;
font-size: 12pt; font-family: 'Times New
Roman', serif;" class=""><b class=""><span
style="font-size: 10pt; font-family:
Tahoma, sans-serif;" class="">From:</span></b><span
style="font-size: 10pt; font-family:
Tahoma, sans-serif;" class=""><span
class="Apple-converted-space"> </span>Jan
Huisken [<a moz-do-not-send="true"
href="mailto:huisken@mpi-cbg.de"
style="color: purple; text-decoration:
underline;" class="">mailto:huisken@mpi-cbg.de</a>]<span
class="Apple-converted-space"> </span><br
class="">
<b class="">Sent:</b><span
class="Apple-converted-space"> </span>Friday,
April 29, 2016 3:01 AM<br class="">
<b class="">To:</b><span
class="Apple-converted-space"> </span>Feinstein,
Timothy N<br class="">
<b class="">Cc:</b><span
class="Apple-converted-space"> </span><a
moz-do-not-send="true"
href="mailto:openspim@openspim.org"
style="color: purple; text-decoration:
underline;" class=""><a class="moz-txt-link-abbreviated" href="mailto:openspim@openspim.org">openspim@openspim.org</a></a><br
class="">
<b class="">Subject:</b><span
class="Apple-converted-space"> </span>Re:
[OpenSPIM] Is anyone using digital
scanning to reduce banding?<o:p class=""></o:p></span></div>
</div>
</div>
<div style="margin: 0in 0in 0.0001pt; font-size:
12pt; font-family: 'Times New Roman', serif;"
class=""><o:p class=""> </o:p></div>
<div style="margin: 0in 0in 0.0001pt; font-size:
12pt; font-family: 'Times New Roman', serif;"
class="">Dear Tim,<o:p class=""></o:p></div>
<div class="">
<div style="margin: 0in 0in 0.0001pt; font-size:
12pt; font-family: 'Times New Roman', serif;"
class=""><o:p class=""> </o:p></div>
</div>
<div class="">
<div style="margin: 0in 0in 0.0001pt; font-size:
12pt; font-family: 'Times New Roman', serif;"
class="">I think you are mixing up two issue.<o:p
class=""></o:p></div>
</div>
<div class="">
<div style="margin: 0in 0in 0.0001pt; font-size:
12pt; font-family: 'Times New Roman', serif;"
class=""><o:p class=""> </o:p></div>
</div>
<div class="">
<div style="margin: 0in 0in 0.0001pt; font-size:
12pt; font-family: 'Times New Roman', serif;"
class=""><b class="">1. Getting rid of
stripes.</b><o:p class=""></o:p></div>
</div>
<div class="">
<div style="margin: 0in 0in 0.0001pt; font-size:
12pt; font-family: 'Times New Roman', serif;"
class=""><o:p class=""> </o:p></div>
</div>
<div class="">
<div style="margin: 0in 0in 0.0001pt; font-size:
12pt; font-family: 'Times New Roman', serif;"
class="">Yes, you can do a little bit in
post-processing but the stripes can be quite
complex and are not necessarily straight.
Oftentimes you see a lot of lensing and I do
not think you can easily remove it with image
processing.<o:p class=""></o:p></div>
</div>
<div class="">
<div style="margin: 0in 0in 0.0001pt; font-size:
12pt; font-family: 'Times New Roman', serif;"
class=""><o:p class=""> </o:p></div>
</div>
<div class="">
<div style="margin: 0in 0in 0.0001pt; font-size:
12pt; font-family: 'Times New Roman', serif;"
class="">a) Double-sided illumination helps.
Depending on the sample you may want to do
simultaneous or sequential double-sided
illumination. There are various ways of
merging the two images.<o:p class=""></o:p></div>
</div>
<div class="">
<div style="margin: 0in 0in 0.0001pt; font-size:
12pt; font-family: 'Times New Roman', serif;"
class="">b) A resonant mSPIM mirror is cheap
and easy to integrate. You will get rid of
most stripes, but again this depends on the
sample. The details for a) and b) are in our
Opt. Lett. paper (Huisken & Stainier,
2007). Basically, you are pivoting the light
sheet around the center of your field of view,
e.g. with 1kHz. The stripes are “washed out”
during the exposure time of your camera. We
use this on most of our systems and it helps a
lot to reduce stripes.<o:p class=""></o:p></div>
</div>
<div class="">
<div style="margin: 0in 0in 0.0001pt; font-size:
12pt; font-family: 'Times New Roman', serif;"
class="">c) Multi-view fusion can also help to
some extent.<o:p class=""></o:p></div>
</div>
<div class="">
<div style="margin: 0in 0in 0.0001pt; font-size:
12pt; font-family: 'Times New Roman', serif;"
class=""><o:p class=""> </o:p></div>
</div>
<div class="">
<div style="margin: 0in 0in 0.0001pt; font-size:
12pt; font-family: 'Times New Roman', serif;"
class=""><b class="">2. DSLM vs. SPIM</b><o:p
class=""></o:p></div>
</div>
<div class="">
<div style="margin: 0in 0in 0.0001pt; font-size:
12pt; font-family: 'Times New Roman', serif;"
class=""><o:p class=""> </o:p></div>
</div>
<div class="">
<div style="margin: 0in 0in 0.0001pt; font-size:
12pt; font-family: 'Times New Roman', serif;"
class="">Yes, DSLM should also help reducing
stripes but I have no experience with that.
Basically, your light sheet is not coherent
anymore and the sheet does not “interfere with
itself”. However, the setup is more expensive
and requires you to power and control another
element: the galvo scanner to sweep the beam
up and down. You need a proper scan mirror and
not just the cheap mSPIM mirror. The
additional benefit is that the resulting light
sheet is more uniform than the cropped
Gaussian light sheet. However, you need to
illuminate each line with higher intensity
which may result in saturation and worse
dynamic range. Obviously you need to wait for
the scan and need to synchronize your readout.
Depending whether you have a global shutter or
rolling shutter camera you need to take some
precaution.<o:p class=""></o:p></div>
</div>
<div class="">
<div style="margin: 0in 0in 0.0001pt; font-size:
12pt; font-family: 'Times New Roman', serif;"
class=""><o:p class=""> </o:p></div>
</div>
<div class="">
<div style="margin: 0in 0in 0.0001pt; font-size:
12pt; font-family: 'Times New Roman', serif;"
class="">Best<o:p class=""></o:p></div>
</div>
<div class="">
<div style="margin: 0in 0in 0.0001pt; font-size:
12pt; font-family: 'Times New Roman', serif;"
class="">Jan<o:p class=""></o:p></div>
</div>
<div class="">
<div style="margin: 0in 0in 0.0001pt; font-size:
12pt; font-family: 'Times New Roman', serif;"
class=""><o:p class=""> </o:p></div>
</div>
<div class="">
<div style="margin: 0in 0in 0.0001pt; font-size:
12pt; font-family: 'Times New Roman', serif;"
class=""><o:p class=""> </o:p></div>
</div>
<div class="">
<div style="margin: 0in 0in 0.0001pt; font-size:
12pt; font-family: 'Times New Roman', serif;"
class=""><o:p class=""> </o:p></div>
<div class="">
<div class="">
<div class="">
<div class="">
<div style="margin: 0in 0in 0.0001pt;
font-size: 12pt; font-family: 'Times
New Roman', serif;" class=""><span
style="" class="">—<o:p class=""></o:p></span></div>
</div>
<div class="">
<div style="margin: 0in 0in 0.0001pt;
font-size: 12pt; font-family: 'Times
New Roman', serif;" class=""><b
class=""><span style="" class="">Jan
Huisken</span></b><span style=""
class=""><o:p class=""></o:p></span></div>
</div>
<div class="">
<div style="margin: 0in 0in 0.0001pt;
font-size: 12pt; font-family: 'Times
New Roman', serif;" class=""><i
class=""><span style="" class="">Head
of Max Planck Research Group</span></i><span
style="" class=""><o:p class=""></o:p></span></div>
</div>
<div class="">
<div style="margin: 0in 0in 0.0001pt;
font-size: 12pt; font-family: 'Times
New Roman', serif;" class=""><span
style="" class=""><o:p class=""> </o:p></span></div>
</div>
<div class="">
<div style="margin: 0in 0in 0.0001pt;
font-size: 12pt; font-family: 'Times
New Roman', serif;" class=""><span
style="" class="">Max Planck
Institute of Molecular Cell Biology
and Genetics (MPI-CBG)<o:p class=""></o:p></span></div>
</div>
</div>
</div>
</div>
<div style="margin: 0in 0in 0.0001pt; font-size:
12pt; font-family: 'Times New Roman', serif;"
class=""><o:p class=""> </o:p></div>
<div class="">
<blockquote style="margin-top: 5pt;
margin-bottom: 5pt;" class="">
<div class="">
<div style="margin: 0in 0in 0.0001pt;
font-size: 12pt; font-family: 'Times New
Roman', serif;" class="">On Apr 27,
2016, at 4:13 PM, Feinstein, Timothy N
<<a moz-do-not-send="true"
href="mailto:tnf8@PITT.EDU"
style="color: purple; text-decoration:
underline;" class="">tnf8@PITT.EDU</a>>
wrote:<o:p class=""></o:p></div>
</div>
<div style="margin: 0in 0in 0.0001pt;
font-size: 12pt; font-family: 'Times New
Roman', serif;" class=""><o:p class=""> </o:p></div>
<div class="">
<div style="margin: 0in 0in 0.0001pt;
font-size: 12pt; font-family: 'Times New
Roman', serif;" class="">Hello all,<span
class="Apple-converted-space"> </span><br
class="">
<br class="">
I found the Selzer group's solution
(Keller et al. 2013) quite interesting<br
class="">
and would love to know if anyone had a
positive experience doing that.<br
class="">
The banding from my basic openSPIM is
definitely a problem for<br class="">
segmentation and analysis.<br class="">
<br class="">
As far as I can tell fixing the banding
will mostly involve buying a scan<br
class="">
mirror. I don't have a quote yet for
the Cambridge VM500 scanning mirror<br
class="">
that the Selzer group used but its
ThorLabs equivalent (#GVS101?) costs<br
class="">
about $1k. That seems reasonable and
not that hard for regular folks to<br
class="">
implement. <br class="">
<br class="">
In related news I just noticed that the
TeraStitcher plugin for Vaa3D now<br
class="">
has an option to fight banding in light
sheet data. I have not tested it<br
class="">
yet but potentially that feature could
be useful. I will just post my<br
class="">
usual warning for folks trying
TeraStitcher for the first time: it<br
class="">
stitches really well but it could be a
hair more user-friendly.<br class="">
<br class="">
Best,<span class="Apple-converted-space"> </span><br
class="">
<br class="">
<br class="">
Tim<br class="">
<br class="">
Timothy Feinstein, Ph.D.<br class="">
Research Scientist<br class="">
University of Pittsburgh Department of
Developmental Biology<br class="">
<br class="">
<br class="">
_______________________________________________<br class="">
OpenSPIM mailing list<br class="">
<a moz-do-not-send="true"
href="mailto:OpenSPIM@openspim.org"
style="color: purple; text-decoration:
underline;" class="">OpenSPIM@openspim.org</a><br
class="">
<a moz-do-not-send="true"
href="http://openspim.org/mailman/listinfo/openspim"
style="color: purple; text-decoration:
underline;" class="">http://openspim.org/mailman/listinfo/openspim</a><o:p
class=""></o:p></div>
</div>
</blockquote>
</div>
<div style="margin: 0in 0in 0.0001pt; font-size:
12pt; font-family: 'Times New Roman', serif;"
class=""><o:p class=""> </o:p></div>
</div>
</div>
</div>
</div>
</span><span style="font-family: Calibri, sans-serif;
font-size: 14px; font-style: normal; font-variant:
normal; font-weight: normal; letter-spacing: normal;
line-height: normal; orphans: auto; text-align: start;
text-indent: 0px; text-transform: none; white-space:
normal; widows: auto; word-spacing: 0px;
-webkit-text-stroke-width: 0px; float: none; display:
inline !important;" class="">_______________________________________________</span><br
style="font-family: Calibri, sans-serif; font-size:
14px; font-style: normal; font-variant: normal;
font-weight: normal; letter-spacing: normal;
line-height: normal; orphans: auto; text-align: start;
text-indent: 0px; text-transform: none; white-space:
normal; widows: auto; word-spacing: 0px;
-webkit-text-stroke-width: 0px;" class="">
<span style="font-family: Calibri, sans-serif; font-size:
14px; font-style: normal; font-variant: normal;
font-weight: normal; letter-spacing: normal;
line-height: normal; orphans: auto; text-align: start;
text-indent: 0px; text-transform: none; white-space:
normal; widows: auto; word-spacing: 0px;
-webkit-text-stroke-width: 0px; float: none; display:
inline !important;" class="">OpenSPIM mailing list</span><br
style="font-family: Calibri, sans-serif; font-size:
14px; font-style: normal; font-variant: normal;
font-weight: normal; letter-spacing: normal;
line-height: normal; orphans: auto; text-align: start;
text-indent: 0px; text-transform: none; white-space:
normal; widows: auto; word-spacing: 0px;
-webkit-text-stroke-width: 0px;" class="">
<a moz-do-not-send="true"
href="mailto:OpenSPIM@openspim.org" style="color:
purple; text-decoration: underline; font-family:
Calibri, sans-serif; font-size: 14px; font-style:
normal; font-variant: normal; font-weight: normal;
letter-spacing: normal; line-height: normal; orphans:
auto; text-align: start; text-indent: 0px;
text-transform: none; white-space: normal; widows: auto;
word-spacing: 0px; -webkit-text-stroke-width: 0px;"
class="">OpenSPIM@openspim.org</a><br
style="font-family: Calibri, sans-serif; font-size:
14px; font-style: normal; font-variant: normal;
font-weight: normal; letter-spacing: normal;
line-height: normal; orphans: auto; text-align: start;
text-indent: 0px; text-transform: none; white-space:
normal; widows: auto; word-spacing: 0px;
-webkit-text-stroke-width: 0px;" class="">
<a moz-do-not-send="true"
href="http://openspim.org/mailman/listinfo/openspim"
style="color: purple; text-decoration: underline;
font-family: Calibri, sans-serif; font-size: 14px;
font-style: normal; font-variant: normal; font-weight:
normal; letter-spacing: normal; line-height: normal;
orphans: auto; text-align: start; text-indent: 0px;
text-transform: none; white-space: normal; widows: auto;
word-spacing: 0px; -webkit-text-stroke-width: 0px;"
class="">http://openspim.org/mailman/listinfo/openspim</a></div>
</blockquote>
</div>
<br class="">
</div>
<br>
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<br>
<pre wrap="">_______________________________________________
OpenSPIM mailing list
<a class="moz-txt-link-abbreviated" href="mailto:OpenSPIM@openspim.org">OpenSPIM@openspim.org</a>
<a class="moz-txt-link-freetext" href="http://openspim.org/mailman/listinfo/openspim">http://openspim.org/mailman/listinfo/openspim</a>
</pre>
</blockquote>
<br>
<div class="moz-signature">-- <br>
<a href="mailto:%20brau@ipmc.cnrs.fr"> Frédéric Brau </a><a> <br>
</a><a href="http://www.ipmc.cnrs.fr"> Institut de Pharmacologie
Moléculaire et Cellulaire - UMR7275 CNRS/UNS </a><a> <br>
660, Route des Lucioles<br>
06560 VALBONNE<br>
Tel: 33 (0)4 93 95 77 83 Bureau / 77 87 / 77 88 / 77 89
Confocaux <br>
Fax: 33 (0)4 93 95 77 08 <br>
</a><a href="http://mica.unice.fr"> Plateforme MICA: Microscopie
Imagerie Côte d'Azur </a><a> <br>
</a><a href="http://rtmfm.cnrs.fr"> Réseau Technologique de
Microscopie de Fluorescence Multidimensionnelle </a><a> <br>
</a></div>
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