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<p class="MsoNormal"><b><span
style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">From:</span></b><span
style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">
Ekaterina PAPUSHEVA
<br>
<b>Sent:</b> Wednesday, June 08, 2016 10:28 AM<br>
<b>To:</b> '<a class="moz-txt-link-abbreviated" href="mailto:openspim@openspim.org">openspim@openspim.org</a>'<br>
<b>Subject:</b> Open SPIM- Cube laser problems<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span style="color:#1F497D">Dear listers,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D">We need a good
advice regarding OpenSPIM laser. We used the Cube, as
recommended. And it died twice by now, after 50-60 hours of
use. Talking with Coherent, we realized that the reason
might lie on the OpenSPIM software. It repeatedly sends
commands “power?" "power=x" at each slice, and this command
seems to wearing out EEPROM of the cube after a million
cycles (which is about 50 hours of continuous imaging…)</span><span
style="font-size:12.0pt;font-family:"Times New
Roman","serif";color:#1F497D"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D">I wonder- are
you familiar with this Cube issue? How do you work around
it? did you remove the "power?" "power=x" commands out of
the OpenSpim software, or do you use other type of laser?<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D">Would be
grateful for any tip!<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D">Katja<o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span
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<p class="MsoNormal"><span
style="font-size:10.5pt;font-family:Consolas"><o:p> </o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.5pt;font-family:Consolas">Ekaterina
Papusheva<o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.5pt;font-family:Consolas"><o:p> </o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.5pt;font-family:Consolas">Manager of
Bioimaging Facility<o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.5pt;font-family:Consolas"><o:p> </o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.5pt;font-family:Consolas">Institute of
Science and Technology Austria<o:p></o:p></span></p>
<p class="MsoNormal"><span
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<p class="MsoNormal"><span
style="font-size:10.5pt;font-family:Consolas">Phone
+43-(0)2243 9000-1043;<o:p></o:p></span></p>
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