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<p>Hi all,</p>
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<p>we have experienced some problems with survival of the larvae when mounted in the FEP tubes. They seems to suffocate after 1-2 h in the tube. Using the same agarose solution but keeping the larvae covered on a glass slide they stay alive and healthy. We
are following the protocol for long time imaging from JOVE (Weber et al 2014) but has also tested 0.5% and 1% low melting agarose solutions for embedding. It would be much appreciated to get some input how to solve this issue.
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<p>Thanks!</p>
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<p>Fredrik</p>
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<p><font size="2" face="Arial">Fredrik Ek, PhD, </font></p>
<p><font size="2"><font face="Arial">Researcher, Chemical Biology & Therapeutics</font><br>
<font face="Arial">Department of Experimental Medical Science </font><br>
<font face="Arial">Lund University, BMC D10 </font><br>
<font face="Arial">S-221 84 Lund, SWEDEN </font><br>
<font face="Arial">------------------------ </font><br>
</font><a href="mailto:Fredrik.Ek@med.lu.se"><font size="2" face="Arial">Fredrik.Ek@med.lu.se</font></a><font size="2"><font face="Arial">
</font><br>
<font face="Arial">Phone: +46-727 17 22 05 </font><br>
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