Loading E:\4dpf_Brain_Imaging_40_beads\3um step, 10x\\spim_TL01_Angle0.tif r1 = 2.0 r2 = 3.0 threshold = 0.010126623325049877 ChannelPattern: Channels: (0) ChannelsToRegister: ChannelsRegister: (0) ChannelsToFuse: ChannelsFuse: (0) (Tue Nov 10 14:51:38 GMT 2015): Creating directory 'E:/4dpf_Brain_Imaging_40_beads/3um step, 10x/output/'. (Tue Nov 10 14:51:38 GMT 2015): Creating directory 'E:/4dpf_Brain_Imaging_40_beads/3um step, 10x/registration/'. Version 0.55 Using model: mpicbg.models.AffineModel3D (Tue Nov 10 14:51:38 GMT 2015): Starting Bead Extraction (Tue Nov 10 14:51:38 GMT 2015): Starting Integral Image based DOM Bead Extraction for spim_TL01_Angle0.tif (Tue Nov 10 14:51:38 GMT 2015): Opening 'E:\4dpf_Brain_Imaging_40_beads\3um step, 10x\spim_TL01_Angle0.tif' [ArrayContainerFactory] (Tue Nov 10 14:51:54 GMT 2015): Computing Integral Image (Tue Nov 10 14:52:02 GMT 2015): min intensity = 0.0, max intensity = 4095.0 (Tue Nov 10 14:52:02 GMT 2015): Computing Difference-of-Mean (Tue Nov 10 14:52:23 GMT 2015): Extracting peaks (Tue Nov 10 14:53:00 GMT 2015): Subpixel localization using quadratic n-dimensional fit Found peaks (possible beads): 637 in view spim_TL01_Angle0.tif (Tue Nov 10 14:53:00 GMT 2015): Starting Integral Image based DOM Bead Extraction for spim_TL01_Angle1.tif (Tue Nov 10 14:53:00 GMT 2015): Opening 'E:\4dpf_Brain_Imaging_40_beads\3um step, 10x\spim_TL01_Angle1.tif' [ArrayContainerFactory] (Tue Nov 10 14:53:18 GMT 2015): Computing Integral Image (Tue Nov 10 14:53:29 GMT 2015): min intensity = 0.0, max intensity = 4095.0 (Tue Nov 10 14:53:29 GMT 2015): Computing Difference-of-Mean (Tue Nov 10 14:53:52 GMT 2015): Extracting peaks (Tue Nov 10 14:54:43 GMT 2015): Subpixel localization using quadratic n-dimensional fit Found peaks (possible beads): 1020 in view spim_TL01_Angle1.tif (Tue Nov 10 14:54:43 GMT 2015): Starting Integral Image based DOM Bead Extraction for spim_TL01_Angle2.tif (Tue Nov 10 14:54:43 GMT 2015): Opening 'E:\4dpf_Brain_Imaging_40_beads\3um step, 10x\spim_TL01_Angle2.tif' [ArrayContainerFactory] (Tue Nov 10 14:54:59 GMT 2015): Computing Integral Image (Tue Nov 10 14:55:07 GMT 2015): min intensity = 0.0, max intensity = 4095.0 (Tue Nov 10 14:55:07 GMT 2015): Computing Difference-of-Mean (Tue Nov 10 14:55:28 GMT 2015): Extracting peaks (Tue Nov 10 14:56:06 GMT 2015): Subpixel localization using quadratic n-dimensional fit Found peaks (possible beads): 859 in view spim_TL01_Angle2.tif (Tue Nov 10 14:56:06 GMT 2015): Starting Integral Image based DOM Bead Extraction for spim_TL01_Angle3.tif (Tue Nov 10 14:56:06 GMT 2015): Opening 'E:\4dpf_Brain_Imaging_40_beads\3um step, 10x\spim_TL01_Angle3.tif' [ArrayContainerFactory] (Tue Nov 10 14:56:22 GMT 2015): Computing Integral Image (Tue Nov 10 14:56:30 GMT 2015): min intensity = 0.0, max intensity = 4095.0 (Tue Nov 10 14:56:30 GMT 2015): Computing Difference-of-Mean (Tue Nov 10 14:56:51 GMT 2015): Extracting peaks (Tue Nov 10 14:57:29 GMT 2015): Subpixel localization using quadratic n-dimensional fit Found peaks (possible beads): 650 in view spim_TL01_Angle3.tif (Tue Nov 10 14:57:29 GMT 2015): Starting Integral Image based DOM Bead Extraction for spim_TL01_Angle4.tif (Tue Nov 10 14:57:29 GMT 2015): Opening 'E:\4dpf_Brain_Imaging_40_beads\3um step, 10x\spim_TL01_Angle4.tif' [ArrayContainerFactory] (Tue Nov 10 14:57:46 GMT 2015): Computing Integral Image (Tue Nov 10 14:57:52 GMT 2015): min intensity = 0.0, max intensity = 4095.0 (Tue Nov 10 14:57:52 GMT 2015): Computing Difference-of-Mean (Tue Nov 10 14:58:12 GMT 2015): Extracting peaks (Tue Nov 10 14:58:50 GMT 2015): Subpixel localization using quadratic n-dimensional fit Found peaks (possible beads): 413 in view spim_TL01_Angle4.tif (Tue Nov 10 14:58:50 GMT 2015): Starting Integral Image based DOM Bead Extraction for spim_TL01_Angle5.tif (Tue Nov 10 14:58:50 GMT 2015): Opening 'E:\4dpf_Brain_Imaging_40_beads\3um step, 10x\spim_TL01_Angle5.tif' [ArrayContainerFactory] (Tue Nov 10 14:59:06 GMT 2015): Computing Integral Image (Tue Nov 10 14:59:14 GMT 2015): min intensity = 0.0, max intensity = 4095.0 (Tue Nov 10 14:59:14 GMT 2015): Computing Difference-of-Mean (Tue Nov 10 14:59:41 GMT 2015): Extracting peaks (Tue Nov 10 15:00:20 GMT 2015): Subpixel localization using quadratic n-dimensional fit Found peaks (possible beads): 419 in view spim_TL01_Angle5.tif Opening files took: 98 sec (19 %) Computation took: 423 sec (81 %) (Tue Nov 10 15:00:20 GMT 2015): Finished Bead Extraction (Tue Nov 10 15:00:20 GMT 2015): Starting Registration spim_TL01_Angle0.tif<->spim_TL01_Angle4.tif: Not enough correspondences found 0, should be at least 12 spim_TL01_Angle3.tif<->spim_TL01_Angle5.tif: Not enough correspondences found 0, should be at least 12 spim_TL01_Angle3.tif<->spim_TL01_Angle4.tif: Not enough correspondences found 0, should be at least 12 spim_TL01_Angle0.tif<->spim_TL01_Angle3.tif: Not enough correspondences found 0, should be at least 12 spim_TL01_Angle0.tif<->spim_TL01_Angle5.tif: Not enough correspondences found 0, should be at least 12 spim_TL01_Angle4.tif<->spim_TL01_Angle5.tif: Not enough correspondences found 0, should be at least 12 spim_TL01_Angle2.tif<->spim_TL01_Angle5.tif: Not enough correspondences found 0, should be at least 12 spim_TL01_Angle0.tif<->spim_TL01_Angle2.tif: Not enough correspondences found 1, should be at least 12 spim_TL01_Angle2.tif<->spim_TL01_Angle4.tif: Not enough correspondences found 0, should be at least 12 spim_TL01_Angle2.tif<->spim_TL01_Angle3.tif: Not enough correspondences found 0, should be at least 12 spim_TL01_Angle0.tif<->spim_TL01_Angle1.tif: Not enough correspondences found 0, should be at least 12 spim_TL01_Angle1.tif<->spim_TL01_Angle5.tif: Not enough correspondences found 0, should be at least 12 spim_TL01_Angle1.tif<->spim_TL01_Angle3.tif: Not enough correspondences found 0, should be at least 12 spim_TL01_Angle1.tif<->spim_TL01_Angle2.tif: Not enough correspondences found 1, should be at least 12 spim_TL01_Angle1.tif<->spim_TL01_Angle4.tif: Not enough correspondences found 0, should be at least 12 spim_TL01_Angle0.tif (id = 0) has 0 correspondences in 0 other views. spim_TL01_Angle1.tif (id = 1) has 0 correspondences in 0 other views. spim_TL01_Angle2.tif (id = 2) has 0 correspondences in 0 other views. spim_TL01_Angle3.tif (id = 3) has 0 correspondences in 0 other views. spim_TL01_Angle4.tif (id = 4) has 0 correspondences in 0 other views. spim_TL01_Angle5.tif (id = 5) has 0 correspondences in 0 other views. The total number of detections was: 3998 The total number of correspondence candidates was: 2 The total number of true correspondences is: 0 Successfully optimized configuration of 0 tiles after 202 iterations: average displacement: ?px minimal displacement: 179,769,313,486,231,570,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000.000px maximal displacement: 0.000px Optimizer Matrices ViewErrorStatistics.setViewSpecificError(): Warning! spim_TL01_Angle0.tif (id = 0) is not part of the structure of my view spim_TL01_Angle0.tif (id = 0) spim_TL01_Angle0.tif (id = 0): is not connected to any other tile! ViewErrorStatistics.setViewSpecificError(): Warning! spim_TL01_Angle1.tif (id = 1) is not part of the structure of my view spim_TL01_Angle1.tif (id = 1) spim_TL01_Angle1.tif (id = 1): is not connected to any other tile! ViewErrorStatistics.setViewSpecificError(): Warning! spim_TL01_Angle2.tif (id = 2) is not part of the structure of my view spim_TL01_Angle2.tif (id = 2) spim_TL01_Angle2.tif (id = 2): is not connected to any other tile! ViewErrorStatistics.setViewSpecificError(): Warning! spim_TL01_Angle3.tif (id = 3) is not part of the structure of my view spim_TL01_Angle3.tif (id = 3) spim_TL01_Angle3.tif (id = 3): is not connected to any other tile! ViewErrorStatistics.setViewSpecificError(): Warning! spim_TL01_Angle4.tif (id = 4) is not part of the structure of my view spim_TL01_Angle4.tif (id = 4) spim_TL01_Angle4.tif (id = 4): is not connected to any other tile! ViewErrorStatistics.setViewSpecificError(): Warning! spim_TL01_Angle5.tif (id = 5) is not part of the structure of my view spim_TL01_Angle5.tif (id = 5) spim_TL01_Angle5.tif (id = 5): is not connected to any other tile! (Tue Nov 10 15:00:20 GMT 2015): Finished Registration Finished processing.