Loading E:\4dpf_Brain_Imaging_20_beads_5step_140exp_3_angles\tiff\\spim_TL01_Angle0.tif r1 = 2.0 r2 = 3.0 threshold = 0.010126623325049877 ChannelPattern: Channels: (0) ChannelsToRegister: ChannelsRegister: (0) ChannelsToFuse: ChannelsFuse: (0) Version 0.55 Using model: mpicbg.models.AffineModel3D (Tue Nov 10 14:17:28 GMT 2015): Starting Bead Extraction (Tue Nov 10 14:17:28 GMT 2015): Starting Integral Image based DOM Bead Extraction for spim_TL01_Angle0.tif (Tue Nov 10 14:17:28 GMT 2015): Opening 'E:\4dpf_Brain_Imaging_20_beads_5step_140exp_3_angles\tiff\spim_TL01_Angle0.tif' [ArrayContainerFactory] (Tue Nov 10 14:17:35 GMT 2015): Computing Integral Image (Tue Nov 10 14:17:39 GMT 2015): min intensity = 0.0, max intensity = 4095.0 (Tue Nov 10 14:17:39 GMT 2015): Computing Difference-of-Mean (Tue Nov 10 14:17:44 GMT 2015): Extracting peaks (Tue Nov 10 14:18:00 GMT 2015): Subpixel localization using quadratic n-dimensional fit Found peaks (possible beads): 4571 in view spim_TL01_Angle0.tif (Tue Nov 10 14:18:01 GMT 2015): Starting Integral Image based DOM Bead Extraction for spim_TL01_Angle1.tif (Tue Nov 10 14:18:01 GMT 2015): Opening 'E:\4dpf_Brain_Imaging_20_beads_5step_140exp_3_angles\tiff\spim_TL01_Angle1.tif' [ArrayContainerFactory] (Tue Nov 10 14:18:15 GMT 2015): Computing Integral Image (Tue Nov 10 14:18:19 GMT 2015): min intensity = 0.0, max intensity = 4095.0 (Tue Nov 10 14:18:19 GMT 2015): Computing Difference-of-Mean (Tue Nov 10 14:18:28 GMT 2015): Extracting peaks (Tue Nov 10 14:18:44 GMT 2015): Subpixel localization using quadratic n-dimensional fit Found peaks (possible beads): 2188 in view spim_TL01_Angle1.tif (Tue Nov 10 14:18:44 GMT 2015): Starting Integral Image based DOM Bead Extraction for spim_TL01_Angle2.tif (Tue Nov 10 14:18:44 GMT 2015): Opening 'E:\4dpf_Brain_Imaging_20_beads_5step_140exp_3_angles\tiff\spim_TL01_Angle2.tif' [ArrayContainerFactory] (Tue Nov 10 14:18:58 GMT 2015): Computing Integral Image (Tue Nov 10 14:19:01 GMT 2015): min intensity = 0.0, max intensity = 4095.0 (Tue Nov 10 14:19:01 GMT 2015): Computing Difference-of-Mean (Tue Nov 10 14:19:12 GMT 2015): Extracting peaks (Tue Nov 10 14:19:31 GMT 2015): Subpixel localization using quadratic n-dimensional fit Found peaks (possible beads): 4981 in view spim_TL01_Angle2.tif Opening files took: 35 sec (29 %) Computation took: 87 sec (71 %) (Tue Nov 10 14:19:32 GMT 2015): Finished Bead Extraction (Tue Nov 10 14:19:32 GMT 2015): Starting Registration spim_TL01_Angle1.tif<->spim_TL01_Angle2.tif: Not enough correspondences found 3, should be at least 12 spim_TL01_Angle0.tif<->spim_TL01_Angle1.tif: Not enough correspondences found 8, should be at least 12 spim_TL01_Angle0.tif<->spim_TL01_Angle2.tif: Not enough correspondences found 8, should be at least 12 spim_TL01_Angle0.tif (id = 0) has 0 correspondences in 0 other views. spim_TL01_Angle1.tif (id = 1) has 0 correspondences in 0 other views. spim_TL01_Angle2.tif (id = 2) has 0 correspondences in 0 other views. The total number of detections was: 11740 The total number of correspondence candidates was: 19 The total number of true correspondences is: 0 Successfully optimized configuration of 0 tiles after 202 iterations: average displacement: ?px minimal displacement: 179,769,313,486,231,570,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000.000px maximal displacement: 0.000px Optimizer Matrices ViewErrorStatistics.setViewSpecificError(): Warning! spim_TL01_Angle0.tif (id = 0) is not part of the structure of my view spim_TL01_Angle0.tif (id = 0) spim_TL01_Angle0.tif (id = 0): is not connected to any other tile! ViewErrorStatistics.setViewSpecificError(): Warning! spim_TL01_Angle1.tif (id = 1) is not part of the structure of my view spim_TL01_Angle1.tif (id = 1) spim_TL01_Angle1.tif (id = 1): is not connected to any other tile! ViewErrorStatistics.setViewSpecificError(): Warning! spim_TL01_Angle2.tif (id = 2) is not part of the structure of my view spim_TL01_Angle2.tif (id = 2) spim_TL01_Angle2.tif (id = 2): is not connected to any other tile! (Tue Nov 10 14:19:32 GMT 2015): Finished Registration Finished processing.