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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Hi Jan, thanks for your insight.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">I haven’t ordered my parts yet, and I’m leaning towards the gimbal for an additional reason. As it’s planned for a core facility I need to keep everything
as covered as possible, and I think the two adjusters on the gimbal will stick through a custom cover quite nicely. This is going on the assumption that this last mirror is used the vast majority of the time compared to the others.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Thanks and enjoy your weekends.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Neil<o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">From:</span></b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif""> Jan Huisken [mailto:huisken@mpi-cbg.de]
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<b>Sent:</b> Thursday, October 08, 2015 6:03 PM<br>
<b>To:</b> Anthony, Neil<br>
<b>Cc:</b> openspim@openspim.org<br>
<b>Subject:</b> Re: [OpenSPIM] ID'ing a part<o:p></o:p></span></p>
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<p class="MsoNormal">Hi Neil,<o:p></o:p></p>
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<p class="MsoNormal">if you already have the 3-axis adjuster, then do not worry about it. I do not think it makes a big difference. I also do not think it is worth buying any fancy mounts that are especially temperature stable. If the mirror is easily accessible
it anyway does not hurt to check the alignment before every experiment...<o:p></o:p></p>
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<p class="MsoNormal">Best<o:p></o:p></p>
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<p class="MsoNormal">Jan<o:p></o:p></p>
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<p class="MsoNormal">On Sep 30, 2015, at 2:34 AM, Anthony, Neil <<a href="mailto:nantho2@emory.edu">nantho2@emory.edu</a>> wrote:<o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Hi Jan, I hope the science is treating you well.</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Could I ask about the gimbal vs. the 3-axis adjuster. How much work is it to remove the tilt using the 3-axis version? I assume you can achieve a pseudo-gimbal
by adjusting a little on two screws at a time…?</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Or maybe, to come at the question from another direction, which would you choose from the two following options?</span><o:p></o:p></p>
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<p class="MsoNormal" style="text-indent:-.25in"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">-</span><span style="font-size:7.0pt;color:#1F497D"> <span class="apple-converted-space"> </span></span><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Polaris
mounts all around, but no gimbal (FYI the room temp variation I see is <2°C most days)</span><o:p></o:p></p>
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<p class="MsoNormal" style="text-indent:-.25in"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">-</span><span style="font-size:7.0pt;color:#1F497D"> <span class="apple-converted-space"> </span></span><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Standard
mirror mounts but a gimbal at that conjugate plane</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Thanks in advance for your time.</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Neil</span><o:p></o:p></p>
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<p class="MsoNormal"><b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">From:</span></b><span class="apple-converted-space"><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif""> </span></span><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif""><a href="mailto:openspim-bounces@openspim.org"><span style="color:purple">openspim-bounces@openspim.org</span></a><span class="apple-converted-space"> </span>[<a href="mailto:openspim-bounces@openspim.org"><span style="color:purple">mailto:openspim-bounces@openspim.org</span></a>]<span class="apple-converted-space"> </span><b>On
Behalf Of<span class="apple-converted-space"> </span></b>Jan Huisken<br>
<b>Sent:</b><span class="apple-converted-space"> </span>Wednesday, August 05, 2015 10:56 AM<br>
<b>To:</b><span class="apple-converted-space"> </span><a href="mailto:openspim@openspim.org"><span style="color:purple">openspim@openspim.org</span></a><br>
<b>Subject:</b><span class="apple-converted-space"> </span>Re: [OpenSPIM] ID'ing a part</span><o:p></o:p></p>
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<p class="MsoNormal"> <o:p></o:p></p>
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<p class="MsoNormal">Hi Tim<o:p></o:p></p>
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<p class="MsoNormal">Correct. This must be a gimbal mirror mount. Sitting in the conjugate plane to the back focal plane you use this mirror to move the light sheet into the focal plane of your detection system (panel C below). If you used a regular (3-screw)
mount you would not only translate the sheet but also introduce a tilt (panel D).<o:p></o:p></p>
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<p class="MsoNormal">This is also illustrated in Fig. 2 of [Huisken, Jan, and Didier Y R Stainier. 2007. “Even Fluorescence Excitation by Multidirectional Selective Plane Illumination Microscopy (mSPIM).” Optics Letters 32 (17): 2608–10.] <o:p></o:p></p>
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<p class="MsoNormal">Best<o:p></o:p></p>
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<p class="MsoNormal">Jan Huisken<o:p></o:p></p>
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<p class="MsoNormal">Figure from Weber, Michael, Michaela Mickoleit, and Jan Huisken. 2014. “Light Sheet Microscopy.” Methods in Cell Biology 123: 193–215. doi:10.1016/B978-0-12-420138-5.00011-2.<o:p></o:p></p>
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<p class="MsoNormal">On Aug 5, 2015, at 4:22 PM, Feinstein, Timothy N <<a href="mailto:tnf8@PITT.EDU"><span style="color:purple">tnf8@PITT.EDU</span></a>> wrote:<o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif"">Thanks folks, I got it. It's a GM100 Ø1" gimbal mirror mount, for adjusting the mirror without changing the axis of rotation. I can see how a POLARIS in that position can
be a little like adjusting cantilever brakes on a bike – lots of axes of adjustment and changing one effects all the others. Having something there that adjusts like 'V' brakes do (independent axes, less frustration) seems like a good idea. </span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif"">All the best, </span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif"">Tim</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif"">Timothy Feinstein, Ph.D. </span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif"">Research Scientist</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif"">University of Pittsburgh Department of Developmental Biology</span><o:p></o:p></p>
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<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri","sans-serif"">From:<span class="apple-converted-space"> </span></span></b><span style="font-size:11.0pt;font-family:"Calibri","sans-serif""><<a href="mailto:openspim-bounces@openspim.org"><span style="color:purple">openspim-bounces@openspim.org</span></a>>
on behalf of Timothy Feinstein <<a href="mailto:tnf8@pitt.edu"><span style="color:purple">tnf8@pitt.edu</span></a>><br>
<b>Date:<span class="apple-converted-space"> </span></b>Wednesday, August 5, 2015 at 9:07 AM<br>
<b>To:<span class="apple-converted-space"> </span></b>"<a href="mailto:openspim@openspim.org"><span style="color:purple">openspim@openspim.org</span></a>" <<a href="mailto:openspim@openspim.org"><span style="color:purple">openspim@openspim.org</span></a>><br>
<b>Subject:<span class="apple-converted-space"> </span></b>[OpenSPIM] ID'ing a part</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif"">Hello, </span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif"">Please forgive the super-basic question. In most diagrams on<span class="apple-converted-space"> </span><a href="http://openspim.org/"><span style="color:purple">openspim.org</span></a><span class="apple-converted-space"> </span>the
last mirror housing before the sample chamber is another POLARIS, but in more advanced designs the mirror housing is a larger and unique item not specified in the basic build list. Can someone let me know what that is? Many thanks and apologies if this is
answered somewhere else, </span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif"">Timothy Feinstein, Ph.D. </span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif"">Research Scientist</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif"">University of Pittsburgh Department of Developmental Biology</span><o:p></o:p></p>
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<p class="MsoNormal">_______________________________________________<br>
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