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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Beautiful! Thanks guys. I’ll keep all these details handy as I get things assembled :)<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Neil<o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">From:</span></b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif""> openspim-bounces@openspim.org [mailto:openspim-bounces@openspim.org]
<b>On Behalf Of </b>Feinstein, Timothy N<br>
<b>Sent:</b> Friday, October 02, 2015 2:47 PM<br>
<b>To:</b> openspim<br>
<b>Subject:</b> [OpenSPIM] Alignment / Calibration Slides<o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black">Date:
</span></b><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black">Friday, October 2, 2015 at 2:16 PM</span><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black">To:
</span></b><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black">Michael Weber <<a href="mailto:weber@mpi-cbg.de">weber@mpi-cbg.de</a>><br>
<b>Subject: </b>Re: [OpenSPIM] Alignment / Calibration Slides<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">Regarding cutting that glass slide, I found the diamond wheel attachment on a Dremel tool to be indispensable for that. I was able to grind the slide fragment
to a skinny trapezoid and wedge it into the opening of a 1 ml syringe without using glue. <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">T <br>
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Timothy Feinstein, Ph.D. <o:p></o:p></span></p>
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<p class="MsoNormal" style="margin-bottom:12.0pt"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black"><br>
On Oct 2, 2015, at 10:22 AM, Michael Weber <<a href="mailto:weber@mpi-cbg.de">weber@mpi-cbg.de</a>> wrote:<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">Dear Neil,
<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">hope you enjoyed reading the paper :). We use a home-made mirror/grid for aligning our setups. The "mirror/grid" part is cut from a stage micrometer:<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">Edmund Optics "Multi-Grid Standard Stage Micrometer"<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black"><a href="http://www.edmundoptics.com/test-targets/image-analysis-test-targets/multi-grid-standard-stage-micrometer/2700/">http://www.edmundoptics.com/test-targets/image-analysis-test-targets/multi-grid-standard-stage-micrometer/2700/</a><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">Cutting the glass is tricky - maybe you have access to a workshop. You want to have both mirror and grid on top of each other on the same piece of glass, such
that you just have to move the sample up and down to switch between them. One end of this piece of glass is then mounted in the tip of a 1 ml Braun Omnifix-F syringe using glue. That's the second difficult step. Ideally, the glass surface ends up straight
in the rotation axis of your setup. Again, if you have access to a workshop, they might be able to help you with that. The syringe is then mounted in the sample holder. Make sure that the syringe has the right length for positioning the glass in the focal
plane. One can also think of a machined or 3D-printed adapter (or entire holder) for the piece of glass.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">Another tool that is really useful for alignment are fluorescent beads / microspheres in 1.5% agarose. This is especially good for the fine tuning of the light
sheet position, PSF measurements and so on. Mirror/grid are more suitable for a coarse alignment.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">Best,<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">Michael<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">On Oct 1, 2015, at 6:35 PM, "Anthony, Neil" <<a href="mailto:nantho2@EMORY.EDU">nantho2@EMORY.EDU</a>> wrote:<o:p></o:p></span></p>
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<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black">Hi all,<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black"> <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black">I was just reading the paper suggested by Jan in Aug (</span><span lang="EN-GB" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black">Weber,
Michael, Michaela Mickoleit, and Jan Huisken. 2014. “Light Sheet Microscopy.” Methods in Cell Biology 123: 193–215. doi:10.1016/B978-0-12-420138-5.00011-2.), thinking ahead to the calibration/alignment part of the setup.</span><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span lang="EN-GB" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black"> </span><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span lang="EN-GB" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black">Does anybody have part numbers and/or websites for the required pieces? Also, I’d be interested in how you’ve positioned and held them at the objective
focus, and ensured their correct orientation.</span><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span lang="EN-GB" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black"> </span><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span lang="EN-GB" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black">Thanks</span><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><span lang="EN-GB" style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black">Neil</span><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:11.5pt;font-family:"Tahoma","sans-serif"">Neil Anthony, PhD</span></b><span style="font-size:11.5pt;font-family:"Tahoma","sans-serif""> | Research Imaging Specialist<br>
</span><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">Health Sciences Research Building (HSRB), Room EG21<br>
1760 Haygood Drive, Atlanta, GA 30322</span><span style="font-size:11.0pt;font-family:"Calibri","sans-serif""><o:p></o:p></span></p>
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<p class="MsoNormal" align="right" style="text-align:right"><span style="font-size:10.5pt;font-family:"Tahoma","sans-serif""><a href="mailto:neil.anthony@emory.edu">neil.anthony@emory.edu</a><br>
<a href="http://www.cores.emory.edu/ici/">ici.emory.edu</a></span><br>
404 969-CORE<span style="font-size:11.0pt;font-family:"Calibri","sans-serif""><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif""><a href="http://www.cores.emory.edu/ici/"><span style="font-size:12.0pt;font-family:"Times New Roman","serif";text-decoration:none"><image001.png></span></a><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif""><a href="https://www.facebook.com/ICIEmory"><span style="font-size:12.0pt;font-family:"Times New Roman","serif";text-decoration:none"><image002.png></span></a><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif""><a href="http://www.linkedin.com/pub/neil-anthony/35/a0b/126"><span style="font-size:12.0pt;font-family:"Times New Roman","serif";text-decoration:none"><image003.png></span></a><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif""><a href="https://www.researchgate.net/profile/Neil_Anthony/"><span style="font-size:12.0pt;font-family:"Times New Roman","serif";text-decoration:none"><image004.png></span></a><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:black"> <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:13.5pt;font-family:"Helvetica","sans-serif";color:black">_____________<br>
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Max Planck Institute of Molecular Cell Biology and Genetics<br>
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