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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Hi Jan, I hope the science is treating you well.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Could I ask about the gimbal vs. the 3-axis adjuster. How much work is it to remove the tilt using the 3-axis version? I assume you can achieve a pseudo-gimbal
by adjusting a little on two screws at a time…?<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Or maybe, to come at the question from another direction, which would you choose from the two following options?<o:p></o:p></span></p>
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</span></span></span><![endif]><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Polaris mounts all around, but no gimbal (FYI the room temp variation I see is <2°C most days)<o:p></o:p></span></p>
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</span></span></span><![endif]><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Standard mirror mounts but a gimbal at that conjugate plane<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Thanks in advance for your time.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Neil<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
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<p class="MsoNormal"><b><span lang="EN-US" style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">From:</span></b><span lang="EN-US" style="font-size:10.0pt;font-family:"Tahoma","sans-serif""> openspim-bounces@openspim.org [mailto:openspim-bounces@openspim.org]
<b>On Behalf Of </b>Jan Huisken<br>
<b>Sent:</b> Wednesday, August 05, 2015 10:56 AM<br>
<b>To:</b> openspim@openspim.org<br>
<b>Subject:</b> Re: [OpenSPIM] ID'ing a part<o:p></o:p></span></p>
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<p class="MsoNormal">Hi Tim<o:p></o:p></p>
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<p class="MsoNormal">Correct. This must be a gimbal mirror mount. Sitting in the conjugate plane to the back focal plane you use this mirror to move the light sheet into the focal plane of your detection system (panel C below). If you used a regular (3-screw)
mount you would not only translate the sheet but also introduce a tilt (panel D).<o:p></o:p></p>
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<p class="MsoNormal">This is also illustrated in Fig. 2 of [Huisken, Jan, and Didier Y R Stainier. 2007. “Even Fluorescence Excitation by Multidirectional Selective Plane Illumination Microscopy (mSPIM).” Optics Letters 32 (17): 2608–10.] <o:p></o:p></p>
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<p class="MsoNormal">Best<o:p></o:p></p>
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<p class="MsoNormal">Jan Huisken<o:p></o:p></p>
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<p class="MsoNormal">Figure from Weber, Michael, Michaela Mickoleit, and Jan Huisken. 2014. “Light Sheet Microscopy.” Methods in Cell Biology 123: 193–215. doi:10.1016/B978-0-12-420138-5.00011-2.<o:p></o:p></p>
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<p class="MsoNormal"><img width="433" height="317" id="_x0036_EDD89A6-2A10-4203-ADCD-72E5300FB18D" src="cid:image001.png@01D0FAF5.7C16E0B0"><o:p></o:p></p>
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<p class="MsoNormal">On Aug 5, 2015, at 4:22 PM, Feinstein, Timothy N <<a href="mailto:tnf8@PITT.EDU">tnf8@PITT.EDU</a>> wrote:<o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif"">Thanks folks, I got it. It's a GM100 Ø1" gimbal mirror mount, for adjusting the mirror without changing the axis of rotation. I can see how a POLARIS in that position can
be a little like adjusting cantilever brakes on a bike – lots of axes of adjustment and changing one effects all the others. Having something there that adjusts like 'V' brakes do (independent axes, less frustration) seems like a good idea. <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif"">All the best, <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif"">Tim<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif""><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif"">Timothy Feinstein, Ph.D. <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif"">Research Scientist<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif"">University of Pittsburgh Department of Developmental Biology<o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri","sans-serif"">From:
</span></b><span style="font-size:11.0pt;font-family:"Calibri","sans-serif""><<a href="mailto:openspim-bounces@openspim.org">openspim-bounces@openspim.org</a>> on behalf of Timothy Feinstein <<a href="mailto:tnf8@pitt.edu">tnf8@pitt.edu</a>><br>
<b>Date: </b>Wednesday, August 5, 2015 at 9:07 AM<br>
<b>To: </b>"<a href="mailto:openspim@openspim.org">openspim@openspim.org</a>" <<a href="mailto:openspim@openspim.org">openspim@openspim.org</a>><br>
<b>Subject: </b>[OpenSPIM] ID'ing a part<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif"">Hello, <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif"">Please forgive the super-basic question. In most diagrams on
<a href="http://openspim.org">openspim.org</a> the last mirror housing before the sample chamber is another POLARIS, but in more advanced designs the mirror housing is a larger and unique item not specified in the basic build list. Can someone let me know
what that is? Many thanks and apologies if this is answered somewhere else, <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif"">Tim<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif"">Timothy Feinstein, Ph.D. <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif"">Research Scientist<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif"">University of Pittsburgh Department of Developmental Biology<o:p></o:p></span></p>
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<p class="MsoNormal">_______________________________________________<br>
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<a href="http://openspim.org/mailman/listinfo/openspim">http://openspim.org/mailman/listinfo/openspim</a><o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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