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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">My response isn’t really simple but…
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">I set up oblique illumination and a camera to aid in sample position after occluding the views of the sample chamber with heating and perfusion lines-and a
sore neck from constantly checking the chamber during positioning. It has worked great for positioning zebrafish to organoids. Right now the illumination is from an old RGB LED board I designed being driven with PWM from a TLC5940 on an Arduino. The Arduino
is also driving an RGB screen for control. The firmware for the Arduino could be adapted to use a customized version of the Arduino micromanager driver-haven’t gotten to it yet. For that matter, I’d like the camera feed to be fed to micromanager too. That’s
all too much development for me right now though. I do have an extra RGB board that is populated and could probably dig up a version of the TLC5940 board that is unpopulated(I might have the parts squirreled away) if you’re interested-firmwares too of course.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Seth<o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">From:</span></b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif""> openspim-bounces@openspim.org [mailto:openspim-bounces@openspim.org]
<b>On Behalf Of </b>Feinstein, Timothy N<br>
<b>Sent:</b> Wednesday, September 02, 2015 8:29 AM<br>
<b>To:</b> openspim@openspim.org<br>
<b>Subject:</b> [OpenSPIM] transmitted light<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">Hello all, <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">Has anyone found a convenient solution for transmitted light illumination that can be controlled Micro-Manager? On the one hand you can use a handheld flashlight
(torch), which works but is not something you can program. On the other hand you have collimated light sources from e.g. Thorlabs. Those do the job but they seem excessively powerful and expensive for what should be a pretty basic task. I went searching
for cheap USB-powered lamps that can be controlled through a software interface rather than a physical switch but those seem strangely hard to find. As it stands I might put a DIY LED bulb in a cheap 'collimator' like the one below and link it to uManager
through an arduino. <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black"><a href="http://www.mouser.com/ds/2/38/V02-1702EN-107315.pdf">http://www.mouser.com/ds/2/38/V02-1702EN-107315.pdf</a><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">Even so I feel like I must have missed a simpler option. Any thoughts appreciated. <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">Thanks and all the best, <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">Tim<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">Timothy Feinstein, Ph.D. <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">Research Scientist<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">University of Pittsburgh Department of Developmental Biology<o:p></o:p></span></p>
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