<html><head><meta http-equiv="Content-Type" content="text/html charset=windows-1252"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">Hi Tim<div class=""><br class=""></div><div class="">Correct. This must be a gimbal mirror mount. Sitting in the conjugate plane to the back focal plane you use this mirror to move the light sheet into the focal plane of your detection system (panel C below). If you used a regular (3-screw) mount you would not only translate the sheet but also introduce a tilt (panel D).</div><div class=""><br class=""></div><div class="">This is also illustrated in Fig. 2 of [Huisken, Jan, and Didier Y R Stainier. 2007. �Even Fluorescence Excitation by Multidirectional Selective Plane Illumination Microscopy (mSPIM).� Optics Letters 32 (17): 2608�10.] <div class=""><br class=""></div><div class="">Best</div><div class="">Jan Huisken</div><div class=""><br class=""></div><div class=""><div class=""><br class=""></div><div class="">Figure from Weber, Michael, Michaela Mickoleit, and Jan Huisken. 2014. �Light Sheet Microscopy.� Methods in Cell Biology 123: 193�215. doi:10.1016/B978-0-12-420138-5.00011-2.</div></div><div class=""><br class=""></div><div class=""><img apple-inline="yes" id="6EDD89A6-2A10-4203-ADCD-72E5300FB18D" height="317" width="433" apple-width="yes" apple-height="yes" src="cid:BA47993C-6994-4CFA-8009-2FF6FAE50D51@mpi-cbg.de" class=""></div><div class=""><div class=""><div class=""><br class=""></div><div class=""><br class=""></div></div><div class=""><br class=""></div><div class=""><br class=""></div><div class=""><br class=""><div><blockquote type="cite" class=""><div class="">On Aug 5, 2015, at 4:22 PM, Feinstein, Timothy N <<a href="mailto:tnf8@PITT.EDU" class="">tnf8@PITT.EDU</a>> wrote:</div><br class="Apple-interchange-newline"><div class="">
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<div class="">Thanks folks, I got it. It's a GM100 �1" gimbal mirror mount, for adjusting the mirror without changing the axis of rotation. I can see how a POLARIS in that position can be a little like adjusting cantilever brakes on a bike � lots of axes of adjustment
and changing one effects all the others. Having something there that adjusts like 'V' brakes do (independent axes, less frustration) seems like a good idea. </div>
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<div class="">All the best, </div>
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<div class="">Timothy Feinstein, Ph.D. </div>
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<div class="">University of Pittsburgh Department of Developmental Biology</div>
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<span style="font-weight:bold" class="">From: </span><<a href="mailto:openspim-bounces@openspim.org" class="">openspim-bounces@openspim.org</a>> on behalf of Timothy Feinstein <<a href="mailto:tnf8@pitt.edu" class="">tnf8@pitt.edu</a>><br class="">
<span style="font-weight:bold" class="">Date: </span>Wednesday, August 5, 2015 at 9:07 AM<br class="">
<span style="font-weight:bold" class="">To: </span>"<a href="mailto:openspim@openspim.org" class="">openspim@openspim.org</a>" <<a href="mailto:openspim@openspim.org" class="">openspim@openspim.org</a>><br class="">
<span style="font-weight:bold" class="">Subject: </span>[OpenSPIM] ID'ing a part<br class="">
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<div class="">Hello, </div>
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<div class="">Please forgive the super-basic question. In most diagrams on <a href="http://openspim.org" class="">openspim.org</a> the last mirror housing before the sample chamber is another POLARIS, but in more advanced designs the mirror housing is a larger and unique item not specified in the basic build
list. Can someone let me know what that is? Many thanks and apologies if this is answered somewhere else, </div>
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<div class="">Timothy Feinstein, Ph.D. </div>
<div class="">Research Scientist</div>
<div class="">University of Pittsburgh Department of Developmental Biology</div>
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