<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1">
</head>
<body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; color: rgb(0, 0, 0); font-size: 14px; font-family: Calibri, sans-serif;">
<div>
<div>
<div>
<div>
<div>
<div>—Apologies for the previous confusion; my new mail program handles things a bit differently than I am used to--</div>
</div>
<div><br>
</div>
<div>Hi Andreas, </div>
<div><br>
</div>
<div>From what I understand MM can work with multi-laser launches but openSPIM currently cannot. I know at least one case where the user could work entirely through MM except that only openSPIM gives you the option to set a settling time after each sample
movement during a stack. If this is the main obstacle to using MM directly, I wonder whether it would be possible to add an extra ‘dummy’ channel with the lasers off, and have the camera acquire in this ‘channel’ for as long as you want the sample to settle.
Just delete the dummy channel at the end of acquisition. It’s a bit of a kludge but it does not call for any coding. </div>
<div><br>
</div>
<div>This question is quite relevant for me as I am getting ready to implement an openSPIM ‘L' design using two lasers in a Versalase-4. </div>
<div><br>
</div>
<div>Best, </div>
<div><br>
</div>
<div><br>
</div>
<div>Tim</div>
<div><br>
</div>
</div>
</div>
<div>
<div>Timothy Feinstein, Ph.D. </div>
<div>Research Scientist</div>
<div>University of Pittsburgh Department of Developmental Biology</div>
<div><br>
</div>
</div>
</div>
</div>
<div><br>
</div>
<div><br>
</div>
<span id="OLK_SRC_BODY_SECTION">
<div style="font-family:Calibri; font-size:11pt; text-align:left; color:black; BORDER-BOTTOM: medium none; BORDER-LEFT: medium none; PADDING-BOTTOM: 0in; PADDING-LEFT: 0in; PADDING-RIGHT: 0in; BORDER-TOP: #b5c4df 1pt solid; BORDER-RIGHT: medium none; PADDING-TOP: 3pt">
<span style="font-weight:bold">From: </span>Andreas GIROD <<a href="mailto:andreas.girod@uni.lu">andreas.girod@uni.lu</a>><br>
<span style="font-weight:bold">Date: </span>Thursday, June 4, 2015 at 6:44 AM<br>
<span style="font-weight:bold">To: </span>OpenSpim Mailinglist <<a href="mailto:openspim@openspim.org">openspim@openspim.org</a>>, "<a href="mailto:fredrik.ek@med.lu.se">fredrik.ek@med.lu.se</a>" <<a href="mailto:fredrik.ek@med.lu.se">fredrik.ek@med.lu.se</a>><br>
<span style="font-weight:bold">Cc: </span>Andreas GIROD <<a href="mailto:andreas.girod@uni.lu">andreas.girod@uni.lu</a>><br>
<span style="font-weight:bold">Subject: </span>Re: [OpenSPIM] multiline openSPIM with Lighthub-4<br>
</div>
<div><br>
</div>
<div>
<div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">
Hi Fredrik,
<div><br>
</div>
<div>are you operating the lighthub with MicroManager / Open SPIM plugin?</div>
<div>I also have an Omicron LightHub-4 with currently only 2 lasers, but the Openspim plugin is not recognizing any of the lasers. Do you have any idea how to get it working?</div>
<div>… In MM the lasers are visible. </div>
<div><br>
</div>
<div>Thanks in advance, </div>
<div><br>
</div>
<div>Andreas<br>
<div apple-content-edited="true"><span class="Apple-style-span" style="border-collapse: separate; font-family: Helvetica; border-spacing: 0px; font-size: medium;"><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; font-size: medium; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; font-size: medium; "><span class="Apple-style-span" style="border-collapse: separate; font-variant: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><span class="Apple-style-span" style="background-color: transparent; "><br>
----------------------------------------------<br>
Dr. Andreas Girod<br>
Coordinator Light Microscopy Facility<br>
Life Sciences Research Unit-FSTC<br>
University of Luxembourg</span></span></span></span>
<div><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; font-size: medium; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; font-size: medium; "><span class="Apple-style-span" style="border-collapse: separate; font-variant: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><span class="Apple-style-span" style="background-color: transparent; ">Building:
Bātiment Des Sciences</span></span></span></span></div>
<div><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; font-size: medium; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; font-size: medium; "><span class="Apple-style-span" style="border-collapse: separate; font-variant: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><span class="Apple-style-span" style="background-color: transparent; ">162A,
ave de la Faiencerie, Campus Limpertsberg<br>
L-1511 Luxembourg<br>
<span></span><span></span><span></span><span></span><span></span><span></span><span></span><span></span><span></span><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; font-size: medium; ">
<p class="MsoNormal"><span lang="EN-US" style="background-color: transparent; "><br class="Apple-interchange-newline">
<br class="Apple-interchange-newline">
Phone Office<span class="Apple-converted-space"> </span> +352 46 66 44 6863<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="background-color: transparent; ">Phone Mobile<span> <span class="Apple-converted-space"> </span></span>+352 62 12 14 889<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="background-color: transparent; ">Fax (office)<span> <span class="Apple-converted-space"> </span></span>+352 46 66 44 6371<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="background-color: transparent; ">FAX (unit office)<span class="Apple-converted-space"> </span><span> <span class="Apple-converted-space"> </span></span>+352 46 66 44 6435<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="background-color: transparent; ">Office<span> <span class="Apple-converted-space"> </span></span>room no<span> <span class="Apple-converted-space"> </span></span>BS 3.08b<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="background-color: transparent; ">Microscope room<span> </span>BS 3.17<o:p></o:p></span></p>
<p class="MsoNormal" style="text-align: justify; "><span lang="EN-US"><span class="Apple-style-span" style="background-color: transparent; ">E-mail :
<a href="mailto:andreas.girod@uni.lu">andreas.girod@uni.lu</a></span><font class="Apple-style-span" face="Arial"><o:p></o:p></font></span></p>
</span></span></span></span></span></div>
</span></div>
<br>
</div>
</div>
</div>
</span>
</body>
</html>