<html><head><meta http-equiv="Content-Type" content="text/html charset=windows-1252"><base href="x-msg://404/"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><br><div><div><br class="Apple-interchange-newline"><blockquote type="cite"><div lang="EN-US" link="blue" vlink="purple" style="font-family: Helvetica; font-size: medium; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><div class="WordSection1" style="page: WordSection1; "><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; ">SPIMers,<br><br>We have completed a multichannel AOTF modulated 4 line L-SPIM. We took an AOTF and three retired lasers(442,488,514,568,647) from our spinning disk and designed a DAC based on the Arduino DAC supported by micromanager (the 442 is not quite integrated optically). We’ve also incorporated an emission filter wheel and excitation wheel for beam characterizing (the former is integrated in our branch of the SPIMAcquisition plugin). I plan to have the optics/hardware/software design and example datasets up on the OpenSPIM site in the coming weeks-grant taking precedent. I’m working with Johannes to get our branch of SPIMAcquisition merged with the release version-waiting on me really.<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; ">On the perfusion and environmental control side, we have a summer student that is working on these aspects for our system. The perfusion is going to be fairly rudimentary. We will be relying on peristaltic pumps because the volume of our sample chamber is so large (7 ml). The perfusion system will be setup to work as a recirculating loop as well to deliver dissolved gases CO2 etc as required-via a simple bubbler. If I can find a CO2 sensor I like(cost to performance) this might be integrated into the bubbler unit.<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; ">I’m not crazy about directly heating the chamber with amped up resistors, so we’re starting with a “closed-loop” heated reservoir, re-circulator and heat-exchanger-if it works well we may also strap a Peltier based heat pump to the reservoir as well to give us cooling.<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; ">In toto there will be a handful of peristaltic pumps, controllers (Arduino as PIDs), media bubblers and heated reservoirs. As time permits we’ll be writing a plugin for micromanager to wrangle all this functionality and add graphs etc-everything you might want out of a comparable Peco or Okolabs solution. <o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; ">As we make progress I’ll update our to-be-completed OpenSPIM page.</div></div></div></blockquote><div><br></div><div>Dear Seth,</div><div><br></div><div>All of the above sounds fantastic. I am very much looking forward to see your wiki page.</div><div><br></div><div>All the best</div><div><br></div><div>PAvel</div><div><br></div><blockquote type="cite"><div lang="EN-US" link="blue" vlink="purple" style="font-family: Helvetica; font-size: medium; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><div class="WordSection1" style="page: WordSection1; "><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "> <o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; ">Happy SPIMing!<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; ">Seth<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div><p class="MsoNormal" style="margin: 0in 0in 12pt; font-size: 12pt; font-family: 'Times New Roman', serif; ">-----Original Message-----<br>From:<span class="Apple-converted-space"> </span><a href="mailto:openspim-bounces@openspim.org" style="color: purple; text-decoration: underline; ">openspim-bounces@openspim.org</a><span class="Apple-converted-space"> </span>[<a href="mailto:openspim-bounces@openspim.org" style="color: purple; text-decoration: underline; ">mailto:openspim-bounces@openspim.org</a>] On Behalf Of Johannes Schindelin<br>Sent: Thursday, May 21, 2015 5:35 AM<br>To: Feinstein, Timothy N<br>Cc:<span class="Apple-converted-space"> </span><a href="mailto:openspim@openspim.org" style="color: purple; text-decoration: underline; ">openspim@openspim.org</a><br>Subject: Re: [OpenSPIM] perfusion and multiple lasers<br><br>Hi Tim,<br><br>On 2015-05-21 03:28, Feinstein, Timothy N wrote:<br><br><o:p></o:p></p><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; ">Separately, If anyone is using openSPIM with two lasers or more I<span class="Apple-converted-space"> </span><br>would be grateful to talk off-list about how you implemented it.<o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><br>I would like to encourage a more open discussion. This is the OpenSPIM project, after all, and it seems pretty obvious to me that the project would benefit more from a discussion *on* this list than from a discussion in private.<br><br>Ciao,<br>Johannes<br><br>_______________________________________________<br>OpenSPIM mailing list<br><a href="mailto:OpenSPIM@openspim.org" style="color: purple; text-decoration: underline; ">OpenSPIM@openspim.org</a><br><a href="http://openspim.org/mailman/listinfo/openspim" style="color: purple; text-decoration: underline; ">http://openspim.org/mailman/listinfo/openspim</a><o:p></o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; "><o:p> </o:p></div></div>_______________________________________________<br>OpenSPIM mailing list<br><a href="mailto:OpenSPIM@openspim.org" style="color: purple; text-decoration: underline; ">OpenSPIM@openspim.org</a><br><a href="http://openspim.org/mailman/listinfo/openspim" style="color: purple; text-decoration: underline; ">http://openspim.org/mailman/listinfo/openspim</a></div></blockquote></div><br></div></body></html>