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<p>Hi everyone,<br>
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<p>This is probably a wider issue with Fiji than OpenSPIM, but I was wondering if anyone else encountered the same problem we are having.<br>
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<p>For reference, we are running 64-bit Windows 7 on a monster machine with 64GB RAM, an Intel Xeon <span style="font-size:12pt">E5-2650 and an nVidia Quadro 5000.</span></p>
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<p>We generally run the 32-bit version of OpenSPIM for acquisition, but opening large image datasets (such as the ones in the Registation tutorial: <a href="http://openspim.org/Registration" id="LPlnk117814">http://openspim.org/Registration</a>) results in
Fiji reaching its memory limit. Because of this, I downloaded the 64-bit version of OpenSPIM/MM/Fiji in an attempt to increase the memory limit. <br>
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<p>If I set the memory limit to a large value (20,000 MB) by going to Edit > Options > Memory & Threads, I get the same error message, as below:<br>
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<div><em>Loading C:\Users\SPIM\Documents\OpenSPIM Dresden Data\tiffs\\spim_TL05_Angle0.tif</em></div>
<div><em>>>>>>>>>>>>>>>>>>>>>>>>>>>></em><br>
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<div><em><Out of memory></em><br>
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<div><em><All available memory (505MB) has been></em></div>
<div><em><used. To make more available, use the></em></div>
<div><em><Edit>Options>Memory & Threads command.></em></div>
<div><em>>>>>>>>>>>>>>>>>>>>>>>>>>>></em></div>
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<div>This is really odd, because if I go to Help > About ImageJ, it says that 13MB out of 20000MB (<1%) is in use. Put another way, Fiji accepts that I want to increase the memory limit, but it still doesn't use more than 505MB of memory.<br>
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<div>As a sanity check, I have installed 64-bit Fiji on my office computer and I can open the Registration tutorial data there, but my personal computer is really slow at manipulating the large datasets!<br>
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<div>Has anyone else experienced this problem? Or does anyone have any suggestions for how to solve the issue?<br>
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<div>It's really frustrating as our behemoth PC refuses to do what it was bought to do!<br>
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<div>Many thanks in advance for any replies.<br>
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<div>Tom Roberts<br>
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<div>Research Associate<br>
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<div>UCL Centre for Advanced Biomedical Imaging<br>
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<div>thomas.roberts.10@ucl.ac.uk<br>
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